Building UI VDL COVID-19 test capacity: evaluating a SARS-CoV-2 LAMP assay for animal samples

项目摘要 SARS-CoV-2大流行已持续两年，造成数百万人感染和死亡。 可能通过最初的人与动物和动物与动物的传播事件，许多动物，如 水貂感染了SARS-CoV-2。SARS-CoV-2已在至少18种动物中检测到， 宠物，圈养，农场，和野生动物。目前还不清楚SARS-CoV-2的起源。的临床试验 是控制新冠肺炎疫情和监测病毒演变的重要步骤之一。分子测试是 通常用于SARS-CoV-2和RT-LAMP是一种用于SARS-CoV-2检测的强大分子检测方法， 它的高特异性和灵敏度，成本效益和快速周转时间。在这份提案中，我们要求 资助伊利诺伊大学兽医诊断实验室（UI VDL）建立检测能力， 用于动物样品的RT-LAMP测试。所有商业化的SARS-CoV-2 RT-LAMP检测试剂盒都是为人类开发的， 因此，需要对动物样品进行RT-LAMP测试的评价。在这个项目中，我们评估 用于在UI VDL中收集的动物样本的商业SARS-CoV-2 RT-LAMP试剂盒。同样的一组积极的 用于RT-LAMP的阴性样品也将通过实时RT-PCR进行检测以进行比较。经验证的 RT-LAMP诊断方法可用于检测不同动物种属样品中的SARS-CoV-2。 因此，验证的RT-LAMP将使UI VDL准备好响应SARS-CoV-2爆发调查 和监视动物。拟议的研究将提高Vet-LIRN实验室的能力， 快速应对新冠肺炎在动物中的爆发，这将对人类和 兽医健康

Project Abstract SARS-CoV-2 pandemic has lasted for two years and caused millions of infections and deaths in humans. Potentially through initial human-to-animal and animal-to animal transmission events, lots of animals such as minks were infected with SARS-CoV-2. SARS-CoV-2 has been detected in at least 18 animal species consisting of pet, captive, farm, and wild animals. It still remains unclear about the origin of SARS-CoV-2. Clinical testing is one of important steps to contain Covid-19 pandemic and monitor the virus evolution. Molecular tests are commonly used for SARS-CoV-2 and RT-LAMP is a powerful molecular assay for SARS-CoV-2 testing because of its high specificity and sensitivity, cost-effectiveness, and fast turnaround time. In this proposal, we request funding to build testing capacity at the University of Illinois Veterinary Diagnostic Laboratory (UI VDL) to perform a RT-LAMP test for animal samples. All commercial SARS-CoV-2 RT-LAMP test kits are developed for human use and there is a need to perform evaluation of RT-LAMP test for animal samples. In this project, we evaluate a commercial SARS-CoV-2 RT-LAMP kit for animal samples collected in the UI VDL. The same set of positive and negative samples used for RT-LAMP will be also tested by real-time RT-PCR for comparison. The validated RT-LAMP diagnostic assay can be used for testing SARS-CoV-2 in samples of different animal species. Therefore, the validated RT-LAMP will make UI VDL ready for responding to SARS-CoV-2 outbreak investigation and surveillance in animals. The proposed study will enhance the capabilities of the Vet-LIRN laboratories to respond quickly to Covid-19 outbreaks in animals, which will have important impacts for both human and veterinary health.