Proteomics Laboratory Shared Resources

摘要/项目摘要 蛋白质组学资源实验室共享资源（PRL） 蛋白质组学资源实验室（PRL）为基于质谱的 所有Perlmutter癌症中心（PCC）研究人员的蛋白质组学分析 该中心拥有先进的仪器设备和高度专业化的技术专长。下 Beatrix Ueberheide博士的指导下， PRL成立于5年前，旨在扩大成功的蛋白质组学， 核心PRL通过提供专业知识来帮助PCC的科学发现， 使用质谱分析蛋白质和肽的最先进仪器 质谱法（MS）。实验室的一个特别重点是协助实验设计， 全面支持下游数据分析，通常涉及创新的数据分析管道。 通过这种资源集中，实现了显著的规模经济。PRL达到 其使命通过四个具体目标：目标1）提供基于质谱的最先进的 为PCC研究人员提供蛋白质组学技术和完整的下游数据分析支持; Aim 2)提供高度定制的支持，针对特定的项目和研究问题， PCC调查员;目标3）开发和实施新技术，以确保持续的 为PCC研究人员提供最先进的蛋白质组学技术支持;目标4）提供 对研究生和博士后研究人员进行蛋白质组学技术的教育 通过研究生教学和与PCC调查人员的密切合作。

Abstract/Project Summary PROTEOMICS RESOURCE LABORATORY SHARED RESOURCE (PRL) The Proteomics Resource Laboratory (PRL) provides support for mass spectrometry-based proteomics analysis for all Perlmutter Cancer Center (PCC) investigators through a centralized facility, with state-of-the-art instrumentation and highly specialized technical expertise. Under the direction of Dr. Beatrix Ueberheide, a well-established expert in biological mass spectromety, PRL was established 5 years ago to expand upon the successful Proteomics Core. PRL aids scientific discovery at PCC by providing access to specialized expertise and state-of-the-art instrumentation for the analysis of proteins and peptides using mass spectrometry (MS). A special focus of the laboratory is assistance with experimental design and full support for downstream data analysis, often involving innovative data analysis pipelines. Significant economies of scale are achieved by this centralization of resources. PRL achieves its mission via four Specific Aims: Aim 1) To provide mass spectrometry-based, state-of-the-art proteomics technologies to PCC investigators and full downstream data analysis support; Aim 2) To provide highly customized support tailored to specific projects and research questions of PCC investigators; Aim 3) To develop and implement novel techniques to ensure continued support of state-of-the-art proteomics technology for PCC investigators; Aim 4) To provide education to graduate students and post-doctoral researchers on proteomics techniques through graduate teaching and close collaboration with PCC investigators.