Light-guide Image Processing (LIP) 3D printed snapshot spectrometer for molecular imaging

用于分子成像的光导图像处理（LIP）3D打印快照光谱仪 本研究的总体目标是开发新一代高性能、紧凑、 与研究级光学显微镜兼容的快照高光谱成像设备。 为了实现这一目标，我们将建立一个光导3D打印成像处理光谱仪。 LIP光谱仪将利用两项创新技术：光纤和双光子添加剂 制造，以允许有效地制造定制光纤束，提供不同的输入与 输出组织。这些自定义包将捕获密集打包的输入， 任意输出，具有允许频谱信息展开的空隙空间。因此，LIP 设备将图像从2D图像（输入）转换为分离的空间-光谱信息（之后 色散）在大幅面sCMOS/CCD图像传感器上。建议的方法不需要 这是一个重要的计算或处理，以创建（x，y，λ）数据立方体。简单的数据重组 将足以创建光谱立方体。 LIP光谱仪将成为一种同时获取全光谱信息的宽视场方法 因此，它将在成像速度和信号方面提供显着的优势 收藏. LIP光谱仪将允许通过不同的方式改变光谱/空间采样。 纤维束和分散器配置。该项目将追求两个具体目标：（1）开发 概念验证LIP光谱仪（250 × 250 × 60，可见光范围470- 670 nm）和（2）测试 LIP光谱仪的动态范围、成像速度和采样率都超过了其它光谱仪 技术，如IMS或扫描方法。我们还将在几个活细胞中进行成像 成像应用。这些实验将侧重于动态生物系统的测试， 特别是研究[ATP]/[ADP]比值，cAMP信号传导和Ca 2+动力学研究博士。 活塞的实验室。我们还将定量评估结果从LIP，对蔡司 LSM 880，Optical Insights光谱DV商用系统和图像映射光谱仪 (IMS)先前由项目PI（Tkaczyk博士）开发。

Light-guide Image Processing (LIP) 3D printed snapshot spectrometer for molecular imaging The overall goal of this research is to develop a new generation of a high performance, compact, snapshot hyperspectral imaging device that is compatible with research grade light microscopes. To accomplish this goal we will build a Light-guide 3D printed Imaging Processing spectrometer. The LIP Spectrometer will leverage two innovative technologies: fiber optics and 2-photon additive manufacturing to allow effective fabrication of custom fiber bundles providing different input vs. output organizations. These custom bundles will capture densely packed input and yield an arbitrary output with void spaces that allow spectral information to be spread out. Thus, the LIP device will convert an image from 2D image (input) to separated spatial-spectral information (after dispersion) on a large format sCMOS/CCDs image sensor. The proposed approach requires no significant computation or processing to create the (x, y, λ) data cube. Simple data re-organization will be sufficient to create spectral cubes. LIP spectrometer will be a widefield method acquiring full spectral information simultaneously from every pixel and therefore it will offer significant advantages in imaging speed and signal collection. The LIP spectrometer will allow changing spectral / spatial sampling through different fiber bundle and disperser configurations. The project will pursue two specific aims: (1) developing a proof-of-concept LIP spectrometer (250x250x60 over visible range 470—670nm) and (2) testing the LIP Spectrometer the dynamic range, imaging speed and sampling over in reference to other techniques like IMS or scanning methods. We will also perform imaging in several living cell imaging applications. These experiments will focus on tests in dynamic biological systems, specifically to study [ATP]/[ADP] ratio, cAMP signaling, and Ca2+ dynamics investigated in Dr. Piston’s lab. We will also quantitatively evaluate the results from the LIP, against the Zeiss LSM880, Optical Insights Spectral DV commercial systems and Image Mapping Spectrometry (IMS) developed previously by project’s PI (Dr.Tkaczyk).