Animal Phenotyping (Core B)

动物表型核心 项目 总结/摘要 啮齿类动物的肥胖模型已被证明对肥胖研究和NYNORC开展的工作是非常宝贵的 investigators.为了满足中心研究人员在评价多个分子和 肥胖症代谢的全身生理指标，包括炎症、基因表达及两者 拟议的NYNORC动物表型核心（APC）反映了颤抖和非颤抖产热 动物能量平衡核心（Einstein）的显著扩展以及与动物成分的整合 脂肪组织核心（哥伦比亚）。自我们上次提交（2015年）以来，APC已处理了10，231个组织 样本和表型分析或为36个NYNORC成员对4023只小鼠/大鼠进行手术，得到31笔赠款的支持， 并为5名NIH资助的非NYNORC成员提供额外服务。APC提供的服务包括： 在获得18项新的或延长的赠款和119项出版物方面发挥了重要作用。根据他们的经验 随着APC核心和高影响力研究期刊不断增长的数据需求，NYNORC成员的 研究计划的回应是扩大他们的复杂性和愿望， 代谢和组织靶向方法的措施在清醒的行为动物。因此，新 14名现有和新的NYNORC成员要求的服务，包括连续血糖监测， 肌电图、体内组织特异性产热和脂肪组织分析导致了4个新的和1个 更新了R 01赠款，并从17个NYNORC用户那里收到了超过250个服务请求， 在下一个供资期间使用这些服务。拟议的APC集中和协调， 扩大了合并后的核心的技术和研究能力。加入额外的动物 在爱因斯坦和哥伦比亚校区的资源，将大大增加用户和研究基地 整个NYNORC，特别是APC核心。通过协调应用纽约市海洋资源中心专用的 设施，资源和专业知识，在哥伦比亚和爱因斯坦，拟议的APC核心结合 对摄食行为进行复杂的定性和定量测量，同时评估 能量消耗、体力活动、体温和可重复的非侵入性身体成分分析 通过MRI和MicroCT。还有一系列的代谢手术，包括胃肠分流术， 自主去神经程序和脂肪组织服务，包括：1）定量脂肪细胞 亚群（棕色，白色，米色），在大小，变化和细胞超微结构方面; 2）初级 细胞分离和分析; 3）脂肪细胞和脂肪组织巨噬细胞的分化;和4） 脂肪组织移植服务范围的扩大源于人们日益认识到， 我们对肥胖生物学的理解需要同时评估能量摄入， 能量消耗，以及确定其对能量平衡的净影响的方法， 个别动物。

Animal Phenotyping Core PROJECT SUMMARY/ABSTRACT Rodent models of obesity have proven invaluable to the study of obesity and to the work carried out by NYNORC investigators. To meet the growing needs of Center researchers in the evaluation of multiple molecular and systemic physiological indices of metabolism in obesity, including inflammation, gene expression, and both shivering and non-shivering thermogenesis, the proposed NYNORC Animal Phenotyping Core (APC) reflects a significant expansion of the Animal Energy Balance Core (Einstein) and integration with the animal component of the Adipose Tissue Core (Columbia). Since our last submission (2015), the APC has processed 10,231 tissue samples and phenotyped or operated on 4023 mice/rats for 36 NYNORC members supported by 31 grants, and provided additional services to 5 NIH-funded non-NYNORC members. The services offered by APC have been instrumental in obtaining 18 new or renewed grants, and in 119 publications. Based on their experiences with the APC core and ever-increasing data demands of high impact research journals, NYNORC members' research programs have responded by expanding in their sophistication and desire for multiple spontaneous measures of metabolism and tissue-targeting approaches in awake behaving animals. Accordingly, new services requested by 14 existing and new NYNORC members, including continuous blood glucose monitoring, electromyography, in vivo tissue-specific thermogenesis, and adipose tissue analyses have led to 4 new and 1 renewed R01 grant, and have generated >250 service requests from 17 NYNORC users for more extensive uses of these services for the next funded period. The proposed APC concentrates and coordinates significantly expanded technical and research capabilities of this amalgamated Core. The incorporation of additional animal resources at both the Einstein and Columbia campuses, will significantly increase the user and research base of the NYNORC overall, and of the APC Core in particular. By coordinated application of NYNORC-dedicated facilities, and resources and expertise at both Columbia and Einstein, the proposed APC Core combines sophisticated qualitative and quantitative measurements of ingestive behavior with concurrent assessments of energy expenditure, physical activity, body temperature, and repeatable noninvasive body composition analysis by MRI and MicroCT. Also available are a range of metabolic surgeries, including gastrointestinal bypass, autonomic denervation procedures, and adipose tissue services including: 1) quantifying adipocyte subpopulations (brown, white, beige), in terms of size, variation, and cellular ultrastructure; 2) primary cell isolation and analysis; 3) differentiation of adipocytes and adipose tissue macrophages; and 4) adipose tissue transplantation. This expanded array of services stems from the growing realization that our understanding of the biology of obesity requires simultaneous assessment of energy intake and energy expenditure, and of the means to determine their net effects on energy balance repeatedly in individual animals.