Molecular Biology/Molecular Genetics (Core C)

MBMG核心 项目总结/摘要 分子生物学/分子遗传学（MBMG）核心服务于NYNORC肥胖研究社区 在过去的5个周期中，这一补助金。MBMG核心为肥胖相关的临床研究提供了关键的研究支持。 分子遗传学、生物信息学和模式生物领域的基础研究活动 特征化这些服务得到广泛使用，提高了NYNORC调查人员的工作效率， 同时降低成本并促进获得先进的基因组学技术。本研究的主要目的 核心是协助研究人员应用分子遗传学和基因组学的工具和技术， 阐明肥胖症的发病机理和医学/生理共病的分子遗传学基础。 MBMG核心的具体目标是：1）促进分子遗传学在以下问题中的应用： 通过提供关于研究设计和适用的分子生物学技术的专家咨询; 2） 为年轻的研究者和没有正式实验室的研究者提供标准的实验室服务， 3）开发和提供新的尖端研究工具和试剂。为此， MBMG核心提供咨询和服务的分子方法，包括基因表达，DNA提取， 基因分型，DNA和RNA测序，通过16 s rRNA进行细菌系统发育表征，RNAscope， 通过SHIELD试剂清除组织、小鼠转基因、干细胞衍生和分化以及基因组 使用CRISPR Cas9进行编辑，以及与这些技术相关的分析工具。核心服务有三种基本类型： 现场直接服务，（二）与机构核心的咨询-合作服务，（三）咨询- 转介。为了获得尖端技术，避免重复，并最大限度地利用我们的 资源，我们与其他本地核心设施和实验室广泛合作。核心人员提供 协助范围从研究设计到研究执行和数据解释。我们强调服务于 年轻的研究人员和P&F的资助者。核心一直积极开发新的研究工具和试剂 并在为我们的社区建立iPS细胞技术和光遗传学方面发挥了重要作用。我们 研发活动的重点是开发用于大脑研究和基因操作的新工具和试剂 在细胞和模型动物中。MBMG核心一直是知识交流和合作的纽带， 是学生、研究员和初级教师的重要培训场所。在上一个周期，核心处理了 来自39个NORC成员的超过20，000个样本，除了10个非成员外，还有43个赠款支持。 这些服务帮助调查人员获得了68项新的赠款和107份出版物。

MBMG Core PROJECT SUMMARY/ABSTRACT The Molecular Biology/Molecular Genetics (MBMG) Core has served the NYNORC obesity research community for the past 5 cycles of this grant. The MBMG Core provides critical research support for obesity-related clinical and basic research activities in the area of molecular genetics, bioinformatics, and model organism characterization. The services are widely used and have increased the productivity of NYNORC investigators, while curtailing costs and facilitating access to advanced genomics technologies. The primary objective of this Core is to assist investigators to apply the tools and technologies of molecular genetics and genomics to elucidate the molecular-genetic basis for the pathogenesis and medical/physiological co-morbidities of obesity. The Specific Aims of the MBMG Core are: 1) To facilitate the application of molecular genetics to problems of obesity by providing expert consultation on both study design and applicable molecular biological techniques; 2) To provide standard laboratory services to young investigators and those without formal labs for studies related to obesity; 3) To develop and make available new cutting edge research tools and reagents. To these ends, the MBMG Core offers consultation and services for molecular methods including gene expression, DNA extraction, genotyping, DNA and RNA sequencing, bacterial phylogenetic characterization by 16s rRNA, RNAscope, clearing of tissue by SHIELD reagents, mouse transgenics, stem cell derivation and differentiation, and genome editing with CRISPR Cas9, and analytic tools related to these techniques. Core services are of 3 basic types: (i) On-site Direct Services, (ii) Consultative-Collaborative services with Institutional Cores, (iii) Consultative- Referral. To gain access to cutting-edge technologies, avoid duplication and to maximally leverage our resources, we collaborate extensively with other local Core facilities and laboratories. Core personnel provide assistance ranging from study design to execution of studies and data interpretation. We emphasize service to young investigators and P&F grantees. The Core has been active in developing new research tools and reagents and has played an essential role in establishing iPS cell technology and optogenetics for our community. Our R&D activities are focused on developing new tools and reagents for brain research and genetic manipulations in cells and model animals. The MBMG Core has been a nexus for intellectual exchange and collaboration, and an important training venue for students, fellows and junior faculty. During the past cycle, the Core processed over >20,000 samples from 39 NORC members supported by 43 grants, in addition to 10 non-members. These services helped investigators generate 68 new grants and 107 publications.