Four-dimensional multi-modality microimaging-microdevice system for high throughput drug screening in vivo

项目摘要 刘贵根博士是一名机械和光学工程师，他的首要职业目标是开发和 转化基于光纤的生物医学光学成像和传感技术。这项研究的题目是“四个-- 用于体内高通量药物筛选的多模式微成像-微器件系统“， 将先进的光学显微成像系统与新兴的微器件相结合，具有巨大的 对药物开发、个性化保健和基础生物医学研究的潜在影响。 候选人：刘医生是哈佛大学布里格姆妇女医院放射科的讲师 医学院。在他之前的博士后培训中，他和同事们开创了一种硅尖光纤- 高速高分辨率的光学传感平台，获得了2015年艾伦·伯曼研究奖 美国海军研究实验室颁发的出版奖。虽然刘博士已经展示了一个成功的轨迹 在工程方面的记录，他在生物医学研究方面的训练是有限的。通过职业发展计划：1) 获得更多双光子荧光和拉曼显微成像的经验；2)学习设计和实现 显微成像-微型设备系统；3)建立体内药物输送和组织反应测试技术；以及 4)提升领导力和职业发展技能，刘博士将在新的领域开始他的独立职业生涯。 导师/环境：刘博士组建了一支强大的导师团队，指导他完成建议的 培训和研究活动。拟议的职业发展计划包括丰富的可用资源 通过布里格姆妇女医院和哈佛医学院，威尔曼的蒂尔尼实验室 光医学中心和麻省理工学院激光生物医学研究中心。 研究：该研究旨在为生物医学建立一个原位多模式光学组织学实验室 微器件，通过四个具体的研究目标：1)实现量化的4D多色双光子 荧光显微成像：2)用四维双光子荧光MI-MD系统进行体内药效试验； 3)开发使用拉曼显微镜的无标记MI-MD系统；以及4)通过 长而灵活的GRIN探头。这些目标的实现将推动微器件朝着潜在的方向迈出一大步 未来的临床采用。 综述：本研究的创新之处在于将3D微成像技术与用于4D的微器件相结合 药物疗效和体内组织反应的测试，将满足高通量药物的迫切需求 放映。候选人已经确定了一组专家，他们提供补充培训和指导 在各个方面都为他完成拟议的研究和发展独立的研究事业。

Project Summary Guigen Liu, Ph.D., is a mechanical and optical engineer whose overarching career goal is to develop and translate optical fiber based biomedical optical imaging and sensing technologies. The research, entitled “Four- dimensional multi-modality microimaging-microdevice system for high throughput drug screening in vivo”, combines the advanced optical microimaging system with an emerging microdevice, which has the huge potential impact on drug development, individualized health care, and fundamental biomedical research. Candidate: Dr. Liu is an Instructor at the Radiology Department of Brigham and Women’s Hospital, Harvard Medical School. During his previous postdoctoral training, he and colleagues pioneered a silicon-tipped fiber- optic sensing platform featuring high speed and high resolution, which earned the 2015 Alan Berman Research Publication Award from the U.S. Naval Research Laboratory. While Dr. Liu has shown a successful track of record in engineering, his training in biomedical research is limited. Through the career development plans: 1) Gain more experience in two-photon fluorescence and Raman microimaging; 2) Learn to design and implement the microimaging-microdevice system; 3) Establish in vivo drug delivery and tissue response testing skills; and 4) Enhance leadership and career development skills, Dr. Liu will launch his independent career in the new field. Mentors/Environment: Dr. Liu has assembled a strong team of mentors to guide him through the proposed training and research activities. The proposed career development plan includes the rich resources available through Brigham & Women’s Hospital and Harvard Medical School, the Tearney Laboratory at the Wellman Center for Photomedicine, and the Laser Biomedical Research Center at Massachusetts Institute of Technology. Research: The research seeks to build an in situ multi-modality optical histological laboratory for the biomedical microdevice, through four specific research aims: 1) To implement quantitative 4D multi-color two-photon fluorescence microimaging; 2) To test drug efficacy in vivo using the 4D two-photon fluorescence MI-MD system; 3) To develop label-free MI-MD system using Raman microscopy; and 4) To investigate microimaging through long and flexible GRIN probes. Completion of these aims will push the microdevice a big step toward potential clinical adoptions in the future. Summary: Innovation of the proposed research is the integration of 3D microimaging and microdevice for 4D testing of drug efficacy and tissue response in vivo, which will meet the pressing needs of high throughput drug screening. The candidate has identified a group of experts who provide complementary training and mentoring on all the aspects for him to complete the proposed research and develop an independent research career.