Cooperative Human Tissue Network Support through Duke's BioRepository & Precision Pathology Center

项目摘要 高质量的生物标本对癌症研究至关重要。该项目利用CAP认证的 杜克生物储存库的基础设施、人力资源、经验和设备， 精密病理学中心（BRPC）主任、团队成员及其附属网络核心 实验室通过国家癌症研究所的 人类组织合作网络（CHTN）。这和生物库的额外CHTN任务最好 做法传播和社区教育通过以下四个具体目标来实现。 目标1：有效地为CHTN研究者提供高质量的组织、血液和液体生物标本， 基础和早期转化研究。通过BRPC及其网络促进标本采集 最佳实践生物储存库，包括洛杉矶的加州大学， 密歇根大学、华盛顿圣路易斯大学、北卡罗来纳州查佩尔山大学、 和芝加哥大学。在杜克和网络位置使用广泛同意， 长期临床注释的最大灵活性。样品处理的最新技术 包括活的标本保存、通过宏观或显微解剖的肿瘤富集、核酸 提取、基因分型、生物标志物表型、全载玻片成像、组织微阵列， 免疫组织化学和原位杂交以及数字图像分析也是可用的。目标二： 提供咨询。杜克病理学家和生物库工作人员为CHTN提供专家咨询 研究人员对人类生物标本的使用、检测方法的选择和样本处理， 适合目的的预分析。目标3：发展和传播关于高质量做法的知识， 生物储存库。这个CHTN团队将承担新的项目，并通过出版物传播成果 并在国家和国际会议上发言。PI将继续在国家和 在生物储存社区中发挥国际领导作用。目标4：教育地方、区域和 在生物医学研究中生物标本的重要性。在当地，杜克队 将扩大社区“生物银行图尔斯”之旅，教育北卡罗来纳州达勒姆人口的重要性， 包括研究中的组织和血液样本，特别是增加样本数量， 来自代表性不足的少数民族。在全国范围内，PI将继续寻求机会， 教育公众和科学界了解拥有充足、高质量、 可供研究的生物标本。例子包括国家播客和会议 面向一般科学研究受众的演讲，并利用印刷品、音频 和面向普通公众的视频新闻，如时事通讯，社交媒体帖子， 采访

PROJECT ABSTRACT High-quality biospecimens are critical to cancer research. This project leverages the CAP-accredited biorepository infrastructure, human resources, experience and equipment of the Duke BioRepository & Precision Pathology Center (BRPC) director, team members and its' affiliated networked core laboratories to provide high-quality biospecimens to investigators through the National Cancer Institute's Cooperative Human Tissue Network (CHTN). This and the additional CHTN missions of biobanking best practice dissemination and community education are addressed through the following four specific aims. Aim 1: Efficiently provide high-quality tissue, blood and fluid biospecimens to CHTN investigators for basic and early translational research. Specimen collection is facilitated through BRPC and its network of best practice biorepositories including those at the University of California at Los Angeles, the University of Michigan, Washington University at St. Louis, University of North Carolina at Chapel Hill, and the University of Chicago. The use of broad consent at Duke and in network locations provides maximum flexibility in long-term clinical annotation. The latest technologies in sample processing including viable specimen preservation, tumor enrichment by macro or microdissection, nucleic acid extraction, genotyping, biomarker phenotyping, whole slide imaging, tissue microarray, immunohistochemistry and in situ hybridization, and digital image analysis are also available. Aim 2: Provide consultation. Duke pathologists and biorepository staff provide expert consultation to CHTN investigators on use of human biospecimens, assay selection and sample processing with an eye toward fit-for-purpose pre-analytics. Aim 3: Develop and disseminate knowledge on high-quality practices for biorepositories. This CHTN team will take on new projects and disseminate results through publication and presentation at national and international meetings. The PI will continue to serve in national and international leadership roles within the biorepository community. Aim 4: Educate the local, regional and national community on the importance of biospecimens in biomedical research. Locally, the Duke team will expand community `biobank tours' to educate the Durham, NC population on the importance of including tissue and blood samples in research and specifically increasing the number of samples available from underrepresented minorities. Nationally, the PI will continue to seek opportunities to educate the public and the scientific community about the importance of having sufficient, high-quality, annotated biospecimens available for research. Examples include national podcasts and meeting presentations geared toward general scientific research audiences and taking advantage of print, audio and video journalism geared toward the lay public such as newsletters, social media postings and interviews.