Cell Preparation and Analysis Core

细胞制备和分析核心 摘要 耶鲁大学的教职员工长期以来一直对非恶性血液病感兴趣 研究。这种兴趣在最近几年进一步加剧，现在包括多个部门和一个 各式各样的学科。细胞准备和分析核心(核心A)最初是在 耶鲁大学血液学卓越合作中心(YCCEH)框架，以满足需求和 耶鲁大学和其他机构的非恶性血液学研究实验室的挑战，通过提供 十二项服务。在过去的四年里，Core A成功地提供了服务和培训， 平均每年约有50名耶鲁用户和10名非耶鲁用户。核心A还有效地培训和/或服务了97 初级研究人员，并为发表30篇论文做出了贡献。随着核心A的成功在过去获得资金 随着时间的推移，我们也越来越意识到非恶性血液学研究的额外需求。我们 因此，建议继续保持现有服务的成功，并纳入新的服务和培训 这是对血液学社区这些额外需求的答案。这16项服务和相关培训 旨在应对血液学实验室面临的挑战，包括(1)相对缺乏获得 原代人类细胞群体和用于研究人类造血的高商业价格，(2)广泛 对难以建立和优化的新兴细胞制备和分析技术感兴趣， (3)需要获取初级患者样本，以及(4)有必要培训初级研究人员进行 血液学基础研究技术严谨，重复性高。核心A将继续被指导 由Diane Krause博士在副主任Jun Lu博士的协助下。此外，加里·库普费尔博士和 Manoj Pillai将是参与核心A服务的关键核心教师。克劳斯、卢、库普费尔和皮莱博士 丰富的血液学研究经验，将用于指导两名研究人员提供有效的 为YCCEH成员和其他NIDDK CCEH联盟的成员提供服务。初级调查员尤其是 鼓励使用核心服务。通过集中血液学关键技术和设施 作为一个通用的培训平台，核心A将帮助各个实验室显著增加研究 提高效率，减少费用，并节省宝贵的调查人员时间。核心的培训方面将允许高效 传播相关技术，防止耶鲁大学成员实验室重复此类工作 在全国范围内。通过维护和提供令人兴奋的新兴技术，Core A实现了 YCCEH的目标是共享创新技术进步，并积极利用这些进步 非恶性血液学研究。

Cell Preparation and Analysis Core ABSTRACT Members of the faculty of Yale University have a longstanding interest in non-malignant hematological research. This interest has further intensified in recent years, now encompassing multiple departments and a variety of disciplines. The Cell Preparation and Analysis Core (Core A) was initially established within the framework of Yale Cooperative Center of Excellence in Hematology (YCCEH) to address the needs and challenges of non-malignant hematology research laboratories both at Yale and in other institutions, by providing twelve services. Over the past four years, Core A has been successful in providing services and training, with an average of ~50 Yale and ~10 non-Yale users annually. Core A also has effectively trained and/or serviced 97 junior researchers, and contributed to the publication of 30 papers. With the success of Core A in the past funding cycle, we have also become increasingly aware of additional needs in non-malignant hematology research. We thus propose to continue the success of existing service offerings and to incorporate new services and training that answer to these additional demands from the hematology community. These 16 services and related training are designed to address challenges facing hematology laboratories, including (1) the relative lack of access to primary human cell populations and high commercial prices for the study of human hematopoiesis, (2) the wide spread interest in emerging cell preparation and analysis techniques which are difficult to establish and optimize, (3) the need to access primary patient samples, and (4) the necessity to train junior researchers to perfrom essential hematology research techniques with rigor and high reproducibility. Core A will continue to be directed by Dr. Diane Krause with the assistance of Associate Director Dr. Jun Lu. Additionally, Drs. Gary Kupfer and Manoj Pillai will be Key Core Faculty participating in Core A services. Drs. Krause, Lu, Kupfer and Pillai have extensive hematology research experience, which will be utilized to direct two research staff to provide effective services to YCCEH members and those in other NIDDK CCEH consortium. Junior investigators are particularly encouraged to use the Core services. Through centralization of key hematology techniques and establishment of a common platform for training, Core A will help individual laboratories to considerably increase research efficiency, reduce expense, and save valuable investigator time. The training aspect of the core will allow efficient dissemination of relevant techniques and prevent redundancy of such efforts in member laboratories at Yale and across the nation. By maintaining and providing exciting new emerging technologies, Core A fulfills the overall goal of YCCEH so that innovative technical advances are shared and positively leveraged for the advancement of non-malignant hematology research.