Project 3 - Lung Health and Inflammation

摘要-项目3：肺健康与炎症 研究表明，青少年吸烟会改变肺部发育，对呼吸产生不利影响 健康，并有助于慢性低度全身炎症与血管的发展有关 疾病和癌症。然而，蒸发的影响还没有得到充分的评估。与其等待，不如 为了全面评估蒸发对疾病发展的风险，我们建议及早确定 青春期蒸发的呼吸、生理和分子效应。鉴于越来越多的 经常吸电子烟的青少年(每月20多天)，更好地了解 蒸发对于为公共卫生传播工作提供信息至关重要。为了解决在知识方面的差距 蒸发的潜在危害，早期蒸发风险的多学科评估(漫威项目) 3将验证这样一种假设，即蒸发早在青春期就损害了呼吸健康，而且蒸发 结果与吸烟暴露和吸烟相关疾病的分子特征重叠 发病机制。具体地说，我们将：1)描述蒸发对青春期呼吸健康的影响； 2)确定与蒸发和呼吸健康结果相关的呼吸道分子变化；以及3) 描述与汽化相关的氧化应激和全身炎症改变。及早发现 在青春期蒸发对呼吸、生理和分子的影响，我们将综合利用 4000多名高中生支气管炎症状的表型特征 调查和重复测量肺功能(圣乔治呼吸问卷和肺活量测定法)和 在两次实验室访问中收集了360名高中生的生物标本(鼻上皮细胞和血液)。 相隔几个月。鼻上皮细胞是下呼吸道支气管上皮细胞的强有力的替代品，并将是 用于检测蒸发对表观基因组和转录组的影响。外周血将被用来 检查汽化对全身炎症的影响。剂量依赖性将通过详细的自我评估 报告、生物标志物(唾液可替宁、TNE、CEMA)和产品表征。从项目中获得的知识 3将通报项目4中与早期呼吸和呼吸疾病有关的健康沟通的发展情况 青春期蒸发对全身炎症的影响。

ABSTRACT – PROJECT 3: Lung Health and Inflammation Research has shown that adolescent cigarette smoking alters lung development, adversely affects respiratory health, and contributes to chronic low-grade, systemic inflammation linked with the development of vascular disease and cancer. However, the effects of vaping have not been fully evaluated. Rather than waiting decades to fully assess the risk of vaping on the development of disease, we propose to characterize early respiratory, physiological, and molecular effects of vaping during adolescence. Given the increasing number of adolescents who vape frequently (20+ days a month), a better understanding of the health consequences of vaping is critical to inform public health communication efforts. To address the gaps in knowledge of the potential harms of vaping, the Multidisciplinary Assessment of Risks of Vaping in Early Life (MARVEL) Project 3 will test the hypothesis that vaping impairs respiratory health as early as adolescence, and that vaping results in overlapping molecular signatures with cigarette smoke exposure and smoking-related disease pathogenesis. Specifically, we will: 1) Characterize the effects of vaping on respiratory health in adolescence; 2) Identify molecular alterations in airways associated with vaping and respiratory health outcomes; and 3) Characterize oxidative stress and systemic inflammatory alterations associated with vaping. To detect early respiratory, physiological, and molecular effects of vaping during adolescence, we will leverage comprehensive phenotypic characterization including bronchitic symptoms of over 4000 high school students from the Online Survey, and repeated measures of lung function (St. George’s Respiratory Questionnaire and spirometry) and collection of biospecimens (nasal epithelial cells and blood) from 360 high school students, at two lab visits 18 months apart. Nasal epithelial cells are robust surrogates for lower airway bronchial epithelial cells, and will be used to examine the effects of vaping on the epigenome and transcriptome. Peripheral blood will be used to examine the effects of vaping on systemic inflammation. Dose-dependency will be assessed by detailed self- report, biomarkers (saliva cotinine, TNE, CEMA) and product characterization. Knowledge gained from Project 3 will inform the development of health communications in Project 4 relevant to the early respiratory and systemic inflammatory effects of vaping during adolescence.