Einstein Mount Sinai Diabetes Research Center

动物生理学核心（APC）采用先进的研究方法，以协助爱因斯坦-芒特 西奈糖尿病研究中心（ES-DRC）的研究人员在体内评估葡萄糖和脂肪酸 代谢、胰岛素敏感性和能量稳态。通过与 在ES-DRC的其他核心中，APC使研究人员能够彻底描述定义的 对葡萄糖和脂质稳态、胰岛素作用的药理学、饮食、环境和遗传改变， 和新陈代谢。为了实现这些目标，动物生理学核心将：1）提供建议和指导， 学生，博士后研究员，研究人员和技术人员在设计和性能的生理 评估糖尿病患者血糖稳态控制和胰岛素作用的必要方法和技术 啮齿类动物，2）向研究者提供全身和组织特异性葡萄糖的专门测量 在啮齿动物模型中的代谢和胰岛素作用，包括胰岛素、胰腺和高血糖钳夹， 自发血糖监测，3）提供专门的胃肠道、神经外科和组织学模型 用于研究胰岛素敏感性、能量平衡、葡萄糖和脂肪酸代谢，包括胃 旁路和脂肪和肝组织去神经支配，成像和光刺激，4）提供整体分析 身体碳水化合物/脂肪酸氧化、能量消耗、产热、食物摄入和自发活动 使用专门的代谢（间接量热法）啮齿动物笼，5）提供饮食影响的评估， 运动、光/暗周期和环境温度对血糖的连续和急性评估 通过肌电图检查体内平衡、代谢和颤抖，6）提供给专门的研究人员 使用磁共振光谱法、microCT测量啮齿动物脂肪组织分布， 作为使用细胞核测量肝脏和肌肉中糖原、肝内脂质和肌细胞内脂质的方法 磁共振（NMR），7）为研究人员提供大脑能量的专门测量， 通过功能性磁共振成像（fMRI）和microPET扫描测定葡萄糖利用率，8）辅助 研究人员在解释数据，并设计进一步的实验方法，以揭示分子 代谢相关啮齿动物表型的生理基础，和9）促进和整合 APC提供的功能评估与其他ES-DRC生物医学核心提供的测定。所有这些 为糖尿病研究的新研究者以及从事糖尿病研究的研究者提供服务， 可以通过使用该核心的专门知识和设施来丰富和扩展的相关项目。

The Animal Physiology Core (APC) employs sophisticated research methodologies to assist Einstein-Mount Sinai Diabetes Research Center (ES-DRC) investigators in the in vivo assessment of glucose and fatty acid metabolism, insulin sensitivity and energy homeostasis in mice and rats. Through collaborative efforts with the other Cores of the ES-DRC, the APC enables investigators to thoroughly characterize the effects of defined pharmacologic, dietary, environmental and genetic alterations on glucose and lipid homeostasis, insulin action, and metabolism. To accomplish these goals, the Animal Physiology Core will: 1) Offer advice and instruction to students, postdoctoral fellows, investigators and technical staff in the design and performance of physiologic approaches and techniques necessary to evaluate the control of glucose homeostasis and insulin action in rodents, 2) Make available to investigators specialized measurements of whole body and tissue-specific glucose metabolism and insulin action in rodent models including insulin, pancreatic and hyperglycemic clamps and spontaneous glucose monitoring, 3) Provide specialized gastrointestinal, neurosurgical and histological models for the study of insulin sensitivity, energy balance, and glucose and fatty acid metabolism, including gastric bypass and adipose and hepatic tissue denervation, imaging and photo-stimulation, 4) Provide analysis of whole body carbohydrate/fatty acid oxidation, energy expenditure, thermogenesis, food intake, and locomotor activity using specialized metabolic (indirect calorimetry) rodent cages, 5) Provide assessments of the effects of diet, exercise, light/dark cycle and environmental temperature on continuous and acute assessments of glucose homeostasis, metabolism, and shivering via electromyography, 6) Make available to investigators specialized measurements of rodent adipose tissue distribution using magnetic resonance spectroscopy, microCT, as well as measurements of glycogen in liver and muscle, intrahepatic lipids and intramyocellular lipids using nuclear magnetic resonance (NMR), 7) Make available to investigators specialized measurements of brain energy and glucose utilization by functional magnetic resonance imaging (fMRI) and microPET scanning, 8) Assist investigators in the interpretation of data and to design further experimental approaches to reveal the molecular and physiological bases of metabolically relevant rodent phenotypes, and 9) Facilitate and integrate the functional assessments provided by the APC with assays provided by other ES-DRC Biomedical Cores. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes- related projects that can be enriched and extended by the use of the expertise and facilities of this Core.