Husbandry and Management Core

项目总结： 维持非人类灵长类SPF繁殖群体需要几个因素来确保动物的 身体和行为健康、养殖生产、遗传多样性和排除特定病原体。这个 辫尾猕猴，M.nomestrina，表现出额外的身体和行为特征， 在蜂群维护方面的额外挑战。新城疫霉菌对致病菌的敏感性更高。 许多微生物的影响。它们对慢病毒的敏感性使它们成为有价值的动物模型 艾滋病毒/艾滋病研究1-11然而，在标准户外繁殖群体中的非人灵长类动物暴露在 环境和媒介传播的病原体，如克氏锥虫和球虫，以及M。 新月形藻暴露在这种环境中，表现出对疾病的更高的敏感性。还必须注意的是 防止新生支原体婴儿体温过低。与恒河猴(M.Mulatta)相反，恒河猴 季节性繁殖，幼虫常年出生，必须在温暖的环境中饲养 环境。 在养殖管理上也存在着不同的问题。马拉塔可以在日本繁殖 在基础设施最少的大型畜栏中，多雄多雌的大型群体。与之形成鲜明对比的是，新月杆菌 在野外12，13形成单个雄性多雌性分裂/融合群，并试图形成多雄性群 造成严重的创伤。即使有稳定的单雄繁育群体，M.的生产力也较低。 14华盛顿国家灵长类研究中心(WaNPRC)有超过50只 对该物种有多年的育种经验，15岁，并拥有满足这些要求的专业知识和资源 挑战。 在这份U42合作协议的支持下，我们将确保SPF新月形分枝杆菌菌落的健康， 包括保持动物没有B病毒、SIV、SRV和STLV，以及环境控制 生物，如克氏毛滴虫和金龟子毛虫。使用适当的生物信息学工具，我们将提高产量 以符合成本效益的方式满足艾滋病毒/艾滋病调查人员的需要。我们将提供 根据美国国立卫生研究院指示的优先顺序，将SPF新月杆菌交给调查人员。我们将扩大预分配 对动物进行克氏毛滴虫、黄病毒、巨细胞病毒和禽流感病毒的筛查。

Project Summary: Maintenance of a non-human primate SPF breeding colony requires several factors to ensure the animals' physical and behavior health, breeding production, genetic diversity, and exclusion of specified pathogens. The pigtailed macaque, M. nemestrina, exhibits additional physical and behavioral characteristics that pose additional challenges in colony maintenance. M. nemestrina exhibit greater susceptibility to the pathogenic effects of many microorganisms. Their susceptibility to lentiviruses makes them valuable animal models for HIV/AIDS research.1-11 However, nonhuman primates in standard outdoor breeding colonies are exposed to environmental and vector-borne pathogens, such as Trypanosoma cruzi and Coccidioides immitis, and M. nemestrina demonstrate enhanced susceptibility to disease with such exposure. Care must also be taken to prevent M. nemestrina infants from hypothermia. In contrast to rhesus macaques (M. mulatta) which exhibit seasonal breeding, infant M. nemestrina are born year-round and must be maintained in a heated environment. Breeding management is also different between M. mulatta and M. nemestrina. M. mulatta can be bred in large multi-male, multi-female groups in large corrals with minimal infrastructure. In contrast, M. nemestrina form single male multi-female fission/fusion groups in the wild12,13 and attempts to form multi-male groups result in significant trauma. Even with stable, single-male breeding groups, productivity is lower for M. nemestrina than for M. mulatta.14 The Washington National Primate Research Center (WaNPRC) has over 50 years of breeding experience with this species,15 and has the expertise and resources to meet these challenges. With support from this U42 cooperative agreement, we will ensure the health of the SPF M. nemestrina colony, including maintaining the animals free of B virus, SIV, SRV, and STLV, along with control of environmental organisms, such as T. cruzi and C. immitis. Using appropriate bioinformatics tools, we will increase production of SPF M. nemestrina to meet the needs of HIV/AIDS investigators in a cost-effective manner. We will provide SPF M. nemestrina to investigators in accordance with NIH-directed priorities. We will expand pre-assignment screening of animals for T. cruzi, flaviruses, CMV, and AAV.