REGULATION OF CAPUT EPIDIDYMIS AND EFFERENT DUCTULES

摘要：不同的人附睾对雄激素的依赖性不同。 附睾区以及雄激素的来源- 血中循环雄激素(CA)与管腔雄激素(LA) 网状液体。假设LA、CA、雌激素或其他 网状液的成分不同地调节结构和 附睾头和附睾头不同亚区的功能 传出小管(ED)。为了检验这一假设，我们建议研究 雄激素受体，雌激素受体，分泌蛋白质的图谱， 在CE和ED各有三个亚区进行组织学检查。三 实验将进行：(1)切除加和不加 睾丸激素植入；(2)睾丸外网状结扎术和不结扎术 睾丸激素植入术；以及(3)睾丸切除加睾酮和 雌激素植入物和雌二醇性植入物。 雄激素和雌激素受体都将通过定量检测 和免疫细胞化学/放射自显影方法。分泌物的概况 蛋白质将通过凝胶电泳法进行分析，其中 组织将在体外与(35S)蛋氨酸孵育。组织学 将包括与以下相关的细胞器的超微结构检查 吸收和蛋白质合成。 鉴于前两个实验的结果将揭示 雄激素，特别是CA和LA之间的区别作用，那些 第三个实验将揭示雌激素的意义。总的来说， 结果将为不同地区的监管提供洞察力 行政长官和勃起功能障碍的分泌物在启动 精子成熟。

ABSTRACT: The epididymal dependence on androgens differs depending upon the region of the epididymis as well as the source of androgens- circulating androgens (CA) in the blood versus luminal androgens (LA) in the rete fluid. It is hypothesized that LA, CA, estrogens, or other components of the rete fluid differentially regulate the structure and function of different sub-regions of the caput epididymis (CE) and efferent ductules (ED). To test the hypothesis, we propose to study androgen receptors, estrogen receptors, profiles of secretory proteins, and histology in three sub-regions each of the CE and ED. Three experiments will be conducted: (1) Orchiectomy with and without testosterone implants; (2) extratesticular rete ligation with and without testosterone implants; and (3) orchiectomy with testosterone and estradiol implants and estradiol implant only. Both androgen and estrogen receptors will be examined with quantitative and immunocytochemical/autoradiographic methods. Profiles of secretory proteins will be analyzed by gel electrophoresis of the medium in which tissues will be incubated in vitro with (35S) methionine. The histology will include ultrastructural examination of organelles associated with absorption and protein synthesis. Whereas results of the first two experiments will reveal the role of androgens, especially the differential role between CA and LA, those of the third experiment will reveal the significance of estrogens. Overall, results will provide insights on the regulation of different regions of the CE and ED whose secretions play an important role in initiation of sperm maturation.