SWITCHING SYSTEMS IN ORAL CANDIDA ALBICANS

Slutsky等人最近的工作，1985年的研究表明， 白色念珠菌具有频繁转换的能力， 许多不同的表型，可以通过 它们的一般菌落形态。 这种切换能力可能会给 C的另一个优势是。白色念珠菌的致病性， 使其对几种常见的抗真菌剂具有抗性。 这 这项研究将检测口腔粘膜C. 白色念珠菌在口腔念珠菌感染部位转换， 免疫功能低下的患者。 将克隆口腔分离株， 将确定主要应变和开关频率。 来自不同表型的DNA的EcoR1探针和来自不同表型的DNA探针 适度重复的元素将用于比较， 建立菌株差异。 糖同化模式将 也可用作鉴定不同表型的手段。 将探讨不同表型的发育行为 在标准实验室条件下， 抗真菌药物的建立。 这些实验应该能确定 可能发生的改变致病性的变化 在表型之间切换时的白色念珠菌。 最后， 希望如果转换确实增加了致病性， 那么就有可能关闭这个“开关” 并确保微生物对抗真菌药物保持敏感 剂

Recent work by Slutsky et al., 1985 has shown that the yeast, Candida albicans has the ability to switch frequently exhibiting a number of different phenotypes which can be distinguished by their general colony morphology. This ability to switch may give an added advantage to C. albicans in its pathogenicity and also render it resistant to several common antifungal agents. This study will examine the ability of oral commensal strains of C. albicans to switch at the site of an oral candidiasis infection in immunocompromised patients. The oral isolates will be cloned and the predominant strain and switch frequency will be determined. EcoR1 digests of DNA from different phenotypes and DNA probes of moderately repetitive elements will be used in a comparison to establish strain differences. Sugar assimilation patterns will also be used as a means of identifying the different phenotypes. Developmental behavior of the different phenotypes will be explored under standard laboratory conditions and their sensitivity to antifungal agents established. These experiments should identify changes which may have occured to alter the pathogenicity of Candida albicans when switching between phenotypes. Ultimately, it is hoped that if the switching does increase the pathogenicity of this organism then it may be possible to turn the "switch off" and ensure that the organism remains sensitive to the antifungal agent.