The design, development and evaluation of a nano/micro filled novel "smart" denta

描述（由申请人提供）：该复合材料系统由新型、无毒、基于硫醇-烯的“智能”聚合物作为其连续相组成，其特征在于低收缩应力、不受空气抑制、低可提取含量和应力的机械性自我报告。该聚合物将全部填充硅烷化二氧化硅颗粒或部分填充纳米至微米尺寸的氟磷灰石晶体，其形状和组成与釉质晶体相似。这些晶体将在上皮/牙髓细胞生物相容性、再矿化能力和抗菌活性方面赋予填料颗粒生物活性。聚合物系统将是自粘性的 以消除对单独粘合剂的需要并降低技术敏感性。复合材料的评价将遵循树脂复合材料最低验收的ISO标准，沿着使用适当细胞系、牙髓干细胞、牙龈成纤维细胞和成骨细胞样细胞进行蛋白水解降解、对口腔致病菌的抗菌活性、离子释放和体外生物相容性的测试技术。Benh的机械性能标记将基于ISO指南和当代临床使用的复合材料的已发表数据，聚合物收缩体积小于8%，复合材料小于RFA中规定的4%体积，但目标为1%体积。该U 01申请的总体目标是生产一种复合材料，该复合材料将使目前牙科实践中使用的复合材料的临床寿命增加一倍。

DESCRIPTION (provided by applicant): This composite system consists of a novel, non-toxic, thiol-ene-based "smart" polymer as its continuous phase, which features low shrinkage stress, not air inhibited, low extractable content, and mechanophoric self- reporting of stress. This polymer will be filled in total with silanated silica particles or in part with fluorapatite crystas of nano to micro size that are similar in shape and composition to enamel crystals. These crystals will impart a bioactivity to the filler particles in the areas of; epithelial/pulp cell biocompatiblity, remineralization capabilities, and antibacterial activity. The polymer system will be self-adhesive to eliminate the need for a separate bonding agent and to reduce technique sensitivity. Evaluation of the composite will follow ISO standards for minimal acceptance for resin composite materials along with testing techniques for proteolytic degradation, antibacterial activity against pathogenic oral bacteria, ion release and in vitro biocompatibility using appropriate cell lines, dental pulp stem cells, gingival fibroblasts and osteoblast-like cells. Benh marking of the mechanical properties will be based on ISO guidelines and published data on contemporary clinically used composites with shrinkage volume of the polymer of less than 8% and the composite less than 4% volume as stated in the RFA but with a goal of 1% volume. The overall objective of this U01 application is to produce a composite that will double the clinical life-time of the composites used in dental practice today.