The design, development and evaluation of a nano/micro filled novel "smart" denta

纳米/微米填充新型“智能”牙科的设计、开发和评估

• 批准号: 8729442
• 负责人: BRIAN H CLARKSON
• 金额: $ 53.8万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8729442
• 关键词: A-factor (Streptomyces); Adhesives; Air; Anti-Bacterial Agents; Apatites; Area; Bacteria; Benchmarking; Biocompatible; Biological; Biological Testing; Biomimetics; Cell Line; Cells; Characteristics; Chemistry; Clinical; Color; Composite Resins; Cues; DNA Sequence Rearrangement; Data; Dental; Dental Enamel; Dental General Practice; Dental Pulp; Dental caries; Dentin; Dentistry; Dentists; Development; Drug Formulations; Ensure; Epithelial; Esthetics; Evaluation; Fibrinogen; Fibroblasts; Filler; Fluorides; Gingiva; Goals; Guidelines; Healed; Hybrids; In Situ; In Vitro; Ions; Lesion; Life; Literature; Longevity; Marketing; Mechanics; Mercury; Modification; Oral cavity; Osteoblasts; Particle Size; Particulate; Patient Self-Report; Patients; Phase; Plant Resins; Polymers; Process; Property; Protocols documentation; Publishing; Relaxation; Reporting; Research; Shapes; Silicon Dioxide; Stem cells; Stress; Sulfhydryl Compounds; System; Techniques; Testing; Time; Tooth structure; Toxic effect; Visible Radiation; allyl sulfide; base; biomaterial compatibility; chromophore; composite restoration; crosslink; design; fluorapatite; healing; improved; meetings; nano; nanosized; novel; oral bacteria; particle; photopolymerization; polymerization; polymerization shrinkage; prototype; public health relevance; remineralization; restoration; restorative dentistry; restorative material; success; surface coating

DESCRIPTION (provided by applicant): This composite system consists of a novel, non-toxic, thiol-ene-based "smart" polymer as its continuous phase, which features low shrinkage stress, not air inhibited, low extractable content, and mechanophoric self- reporting of stress. This polymer will be filled in total with silanated silica particles or in part with fluorapatite crystas of nano to micro size that are similar in shape and composition to enamel crystals. These crystals will impart a bioactivity to the filler particles in the areas of; epithelial/pulp cell biocompatiblity, remineralization capabilities, and antibacterial activity. The polymer system will be self-adhesive to eliminate the need for a separate bonding agent and to reduce technique sensitivity. Evaluation of the composite will follow ISO standards for minimal acceptance for resin composite materials along with testing techniques for proteolytic degradation, antibacterial activity against pathogenic oral bacteria, ion release and in vitro biocompatibility using appropriate cell lines, dental pulp stem cells, gingival fibroblasts and osteoblast-like cells. Benh marking of the mechanical properties will be based on ISO guidelines and published data on contemporary clinically used composites with shrinkage volume of the polymer of less than 8% and the composite less than 4% volume as stated in the RFA but with a goal of 1% volume. The overall objective of this U01 application is to produce a composite that will double the clinical life-time of the composites used in dental practice today.

描述(申请人提供)：该复合体系由一种新型的、无毒的、基于硫醇-烯的“智能”聚合物作为其连续相组成，其特点是低收缩应力、不受空气抑制、低可萃取物含量以及机械能自我报告应力。这种聚合物将全部填充硅烷化二氧化硅颗粒或部分填充纳米到微米尺寸的氟磷灰石晶体，这些晶体在形状和组成上与釉质晶体相似。这些晶体将赋予填料颗粒在以下方面的生物活性：上皮/牙髓细胞的生物相容性、再矿化能力和抗菌活性。聚合物系统将是自粘性的 消除了对单独粘结剂的需要，并降低了技术敏感性。该复合材料的评估将遵循ISO树脂复合材料的最低可接受性标准，以及使用适当的细胞系、牙髓干细胞、牙龈成纤维细胞和成骨细胞进行蛋白质降解、对口腔致病菌的抗菌活性、离子释放和体外生物兼容性的测试技术。BENH机械性能的标记将基于ISO指南和当代临床使用的聚合物收缩体积小于8%的复合材料以及RFA中规定的复合材料体积小于4%但目标为1%的数据。这一U01应用的总体目标是生产一种复合材料，它将使目前牙科实践中使用的复合材料的临床寿命翻一番。