The design, development and evaluation of a nano/micro filled novel "smart" denta

纳米/微米填充新型“智能”牙科的设计、开发和评估

• 批准号: 8610716
• 负责人: BRIAN H CLARKSON
• 金额: $ 49.67万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8610716
• 关键词: A-factor (Streptomyces); Adhesives; Air; Anti-Bacterial Agents; Apatites; Area; Bacteria; Benchmarking; Biocompatible; Biological; Biological Testing; Biomimetics; Cell Line; Cells; Characteristics; Chemistry; Clinical; Color; Composite Resins; Cues; DNA Sequence Rearrangement; Data; Dental; Dental Enamel; Dental General Practice; Dental Pulp; Dental caries; Dentin; Dentistry; Dentists; Development; Drug Formulations; Ensure; Epithelial; Esthetics; Evaluation; Fibrinogen; Fibroblasts; Filler; Fluorides; Gingiva; Goals; Guidelines; Healed; Hybrids; In Situ; In Vitro; Ions; Lesion; Life; Literature; Longevity; Marketing; Mechanics; Mercury; Modification; Oral cavity; Osteoblasts; Particle Size; Particulate; Patient Self-Report; Patients; Phase; Plant Resins; Polymers; Process; Property; Protocols documentation; Publishing; Relaxation; Reporting; Research; Shapes; Silicon Dioxide; Stem cells; Stress; Sulfhydryl Compounds; System; Techniques; Testing; Time; Tooth structure; Toxic effect; Visible Radiation; allyl sulfide; base; biomaterial compatibility; chromophore; composite restoration; crosslink; design; fluorapatite; healing; improved; meetings; nano; nanosized; novel; oral bacteria; particle; photopolymerization; polymerization; polymerization shrinkage; prototype; public health relevance; remineralization; restoration; restorative dentistry; restorative material; success; surface coating

Abstract This composite system consists of a novel, non-toxic, thiol-ene-based "smart" polymer as its continuous phase, which features low shrinkage stress, not air inhibited, low extractable content, and mechanophoric self- reporting of stress. This polymer will be filled in total with silanated silica particles or in part with fluorapatite crystals of nano to micro size that are similar in shape and composition to enamel crystals. These crystals will impart a bioactivity to the filler particles in the areas of; epithelial/pulp cell biocompatibility, remineralization capabilities, and antibacterial activity. The polymer system will be self-adhesive to eliminate the need for a separate bonding agent and to reduce technique sensitivity. Evaluation of the composite will follow ISO standards for minimal acceptance for resin composite materials along with testing techniques for proteolytic degradation, antibacterial activity against pathogenic oral bacteria, ion release and in vitro biocompatibility using appropriate cell lines, dental pulp stem cells, gingival fibroblasts and osteoblast-like cells. Bench marking of the mechanical properties will be based on ISO guidelines and published data on contemporary clinically used composites with shrinkage vol. of the polymer of less than 8% and the composite less than 4% vol. as stated in the RFA but with a goal of 1% vol. The overall objective of this U01 application is to produce a composite that will double the clinical life-time of the composites used in dental practice today.

摘要 这种复合体系由一种新型的、无毒的、基于硫醇-烯的“智能”聚合物组成，作为其连续的 相，收缩应力低，不受空气抑制，萃取物含量低，具有机械自发性。 关于压力的报告。这种聚合物将全部填充硅烷化二氧化硅颗粒或部分填充氟磷灰石 在形状和成分上与釉质晶体相似的纳米到微米尺寸的晶体。这些水晶会 赋予填充物颗粒生物活性；上皮/牙髓细胞生物相容性，再矿化 能力和抗菌活性。聚合物系统将是自粘性的，以消除对 分离粘结剂，降低工艺敏感性。对复合材料的评估将遵循ISO 树脂复合材料最小可接受性标准及蛋白质分解试验技术 降解、对口腔致病菌的抗菌活性、离子释放和体外生物相容性 使用合适的细胞系、牙髓干细胞、牙龈成纤维细胞和成骨细胞样细胞。长凳 机械性能的标记将基于ISO指南和当代发布的数据 临床上使用的收缩复合材料卷。低于8%的聚合物和低于4%的复合材料 卷。正如RFA中所述，但目标是1%VOL。该U01应用程序的总体目标是生成一个 这种复合材料将使目前牙科实践中使用的复合材料的临床寿命翻一番。