The design, development and evaluation of a nano/micro filled novel "smart" denta

摘要 这种复合系统由一种新型的、无毒的、基于硫醇-烯的“智能”聚合物组成， 低收缩应力、不受空气抑制、可萃取物含量低、机械性能好， 报告压力。该聚合物将全部填充硅烷化二氧化硅颗粒或部分填充氟磷灰石 纳米至微米尺寸的晶体，其形状和组成与釉质晶体相似。这些水晶 赋予填料颗粒在以下领域的生物活性：上皮/牙髓细胞生物相容性， 能力和抗菌活性。聚合物系统将是自粘性的，以消除对粘合剂的需要。 分离粘结剂，降低工艺敏感性。复合材料的评价将遵循ISO 树脂复合材料最低验收标准沿着蛋白水解试验技术 降解、对口腔致病菌的抗菌活性、离子释放和体外生物相容性 使用合适的细胞系、牙髓干细胞、牙龈成纤维细胞和成骨细胞样细胞。板凳 机械性能的标记将基于ISO指南和当代已公布的数据， 聚合物收缩体积小于8%，复合材料收缩体积小于4%的临床用复合材料 卷如RFA中所述，但目标为1% vol。本U 01申请的总体目标是生产 这种复合材料将使目前牙科实践中使用的复合材料的临床寿命增加一倍。

Abstract This composite system consists of a novel, non-toxic, thiol-ene-based "smart" polymer as its continuous phase, which features low shrinkage stress, not air inhibited, low extractable content, and mechanophoric self- reporting of stress. This polymer will be filled in total with silanated silica particles or in part with fluorapatite crystals of nano to micro size that are similar in shape and composition to enamel crystals. These crystals will impart a bioactivity to the filler particles in the areas of; epithelial/pulp cell biocompatibility, remineralization capabilities, and antibacterial activity. The polymer system will be self-adhesive to eliminate the need for a separate bonding agent and to reduce technique sensitivity. Evaluation of the composite will follow ISO standards for minimal acceptance for resin composite materials along with testing techniques for proteolytic degradation, antibacterial activity against pathogenic oral bacteria, ion release and in vitro biocompatibility using appropriate cell lines, dental pulp stem cells, gingival fibroblasts and osteoblast-like cells. Bench marking of the mechanical properties will be based on ISO guidelines and published data on contemporary clinically used composites with shrinkage vol. of the polymer of less than 8% and the composite less than 4% vol. as stated in the RFA but with a goal of 1% vol. The overall objective of this U01 application is to produce a composite that will double the clinical life-time of the composites used in dental practice today.