A novel desensitizing paste containing enamel-like crystals

描述（由申请人提供）：牙本质过敏（DH）是一种常见的问题，治疗方法即使有效，也是短期的。本项目的目标是生产一种能够堵塞未闭牙本质小管（造成DH的原因）并粘附在牙本质小管潮湿的内表面的产品。本研究的目的是优化氟磷灰石（FA）晶体/粘接剂聚合物、聚合物（DMA-MEA）的比例，用于牙本质小管未闭的即时和长期咬合。FA晶体与牙釉质和合成聚合物中发现的晶体相同，合成聚合物模仿了贻贝黏液中发现的裸氨基酸3,4二羟基苯丙氨酸（DOPA）。将合成的FA晶体与聚合物按一定比例混合，应用于牙本质切片并测量牙本质电导率（用于测试牙本质脱敏剂的金标准）。一旦确定了最佳的咬合比例，将在扫描电镜中观察其对牙本质小管咬合的影响。从FA/聚合物膏体中释放的F、Ca和PO4也将在中性和酸性ph值下进行测量。这种离子释放与唾液一起，将有助于在牙本质上形成矿化层，这将有助于长期缓解DH。该产品用于治疗DH将是：生物相容性，无毒，允许离子扩散；轻松地应用;在口腔潮湿的环境中粘附牙齿结构；美学——一种“天然”产品。

DESCRIPTION (provided by applicant): Dentin hypersensitivity (DH) is a common problem and the treatment modalities are, if they work at all, short lived. The object of this project is t produce a product that can occlude patent dentinal tubules (the cause of DH) and adhere to the wet interior surface of the dentinal tubules. The research goal of this study is to optimize the fluorapatite (FA) crystal/polymer of adhesive polymer, poly(DMA-MEA) ratio for immediate and long term occlusion of the patent dentinal tubules. The FA crystals are the same as those found in dental enamel and synthetic polymer which mimics the naked amino acid 3,4 dihydroxyphenylalanine (DOPA) found in the mussel adhesive mucus. The synthesized FA crystals will be mixed with the polymer at certain ratios, applied to dentin sections and the dentinal conductivity (the gold standard used for testing dentin desensitizing agents) measured. . Once the optimized ratio for occlusion has been established its effect on dentinal tubule occlusion will be observed in the SEM . The release of F, Ca and PO4 from the FA/polymer paste will also be measured at neutral and acidic pH. This ion release, along with saliva, will help in the formation of a mineralized layer on the dentin which will help in the long term relief f DH. This product for treating DH will be: biocompatible, non-toxic, allow for diffusion of ions; easily applied; adherent to tooth structure in the wet environment of the oral cavity; and aesthetic - a "natural" product. PUBLIC HEALTH RELEVANCE: This grant proposes a novel way of treating sensitive teeth; a problem that affects about 50% of the population at sometime. A desensitizing paste will be produced which contains mineral crystals which are similar to the natural crystals in teeth, mixed with a gel that will stick to wet tooth surfaces. It is anticipated that the paste will give immedite relief by blocking open tubules running from the surface of the dentin to the live part of the toot; and long term relief by causing a mineralized barrier to form, overtime, on the sensitive tooth surface.