WORKSHOP--SITE-SPECIFIC RECOMBINATION & TRANSPORTATION

要求为特定地点的简化问题讲习班提供部分支助 1990年9月12日至16日在海军陆战队举行 位于马萨诸塞州伍兹霍尔的生物实验室。 本次研讨会的目的是收集讨论一个选定的，但 一群有代表性的科学家，他们积极致力于 位点特异性的生化机制的研究前沿 重组和转座。这组相互关联的反应 在许多非常重要的生物过程的核心： 整合逆转录病毒，包括那些负责艾滋病和 某些形式的癌症，抗体多样性的产生， 抗生素抗性基因，细菌染色体重排， 真核内含子的迁移率。 本次研讨会的口头报告将强调机械性 这些重组反应。领导人专门会议 这一领域的工作应该是及时和富有成效的。已经有迅速的 在许多不同的系统中取得了新的进展。此外，它正在成为 越来越明显的是，发生在真核生物中的反应， 与那些在细菌中发现的密切相关：艾滋病的整合 病毒和其他逆转录病毒在机制上密切相关， 整合细菌转座子、位点特异性免疫球蛋白 重排与原核位点特异性 重组反应等。在促进交流的见解和 从不同的系统，工作坊应大大提高我们的 对所有这些反应的基本理解。另外在 特别是艾滋病病毒和其他逆转录病毒的情况下，它应该进一步 利用重组反应作为靶向治疗的目的 干预 发言者名单上包括应用生物化学， 遗传和拓扑分析，包括几个谁已经推动了 重组分析首次进入酶的领域 动力学主题将包括逆转录病毒的整合， 整合酶和解离酶家族，霍利迪连接，免疫球蛋白 重组，原核转座因子，和果蝇P 元素 我们还鼓励从事以下工作的科学家展示海报： 这些系统还不能进行机械分析。这应该 既扩大会议的生物学基础，又鼓励 那些在相对不发达的系统中工作的人， 机械的方法。 会议将限于100-135名与会者。这个数字 足以包括所有正在工作的主要研究人员 在这一领域富有成效，并对博士后进行合理抽样 研究员和偶尔优秀的研究生。但这 数字足够小，以便在所有 参与者仍然可以。

Partial support is requested for a Workshop on Site-specific Recombination and Transposition to be held on September 12-16, 1990, at the Marine Biological Laboratory in Woods Hole, Massachusetts. The purpose of this workshop is to assemble for discussions a select but representative group of scientists who are actively working at the forefront of research on the biochemical mechanisms for site-specific recombination and transposition. This interrelated group of reactions lie at the heart of a number of very important biological processes: integration of retroviruses including those responsible for AIDS and certain forms of cancer, generation of antibody diversity, transmission of antibiotic resistance genes, rearrangement of bacterial chromosomes, and mobility of eukaryotic introns. The oral presentations at this Workshop will emphasize the mechanistic aspects of these recombination reactions. A specialized meeting for leaders in this field should be both timely and productive. There has been rapid recent progress in many different systems. Furthermore, it is becoming increasingly apparent that reactions occurring in eukaryotic organisms are intimately related to those identified in bacteria: integration of AIDS virus and other retroviruses is mechanistically closely related to integration of bacterial transposons, site specific immunoglobulin rearrangements are closely related to prokaryotic site specific recombination reactions, etc. In promoting the exchange of insights and information from different systems, the Workshop should greatly enhance our basic understanding of all of these reactions. In addition, in the particular case of AIDS virus and other retroviruses, it should further the goal of using the recombination reaction as a target therapeutic intervention. The roster of speakers includes scientists who have applied biochemical, genetic and topological analysis including several who have pushed the analysis of recombination for the first time into the realm of enzyme kinetics. Topics will include retroviral integration, reactions of the integrase and resolvase families, Holliday junctions, immunoglobulin rearrangements, prokaryotic transposable elements, and Drosophila P elements. We are also encouraging poster presentations from scientists working in systems which are not yet amenable to mechanistic analysis. This should serve both to broaden the biological base of the meeting and to encourage those working in relatively undeveloped systems to undertake a more mechanistic approach. The meeting will be limited to 100-135 participants. This number is sufficient to include all of the principal investigators who are working productively in this area as well as a reasonable sampling of postdoctoral fellows and an occasional outstanding graduate student. However, this number is small enough that efficient interchange amongst all of the participants is still possible.