The Dictyostelium Stock Center

描述（由申请人提供）：简单的真核生物盘状盘基骨柱（Dictyostelium disideum）是一种模式生物，广泛用于研究细胞运动、趋化性、细胞分化、信号转导、人类疾病的分子基础、药物反应和耐药的细胞机制等细胞过程。作为一种必须与其他微生物竞争和保护自己的土壤居民，它会产生大量的次生代谢物，这些代谢物可能会引起医学上的兴趣。它能够从与它有不同程度遗传关系的其他细胞中识别自己，这使它成为研究微生物生态系统中自我/非自我识别的一个迷人的模型。此外，它在植物和动物之间的系统发育位置使其成为比较基因组学研究中有用的生物。所有这些研究都需要使用菌株，无论是野生型变种还是转基因菌株。自2002年以来，盘基骨门研究界一直受益于中央库存中心的存在。这种资源提供了一种具有成本效益的方法来确保菌株的可用性并保持在标准化的条件下。股票中心目前由Richard Kessin和Jakob Franke（哥伦比亚大学）监管，他们将在当前融资期结束时退休。该应用程序寻求支持继续收集已知的社会变形虫物种和分离的充分研究的物种，如D. discoideum和D. purpureum，以及具有有趣表型特征的转基因菌株。这提供了一个很好的机会，使库存中心和盘状盘齿龙基因组资源dictyBase更接近。西北大学是dictyBase的所在地，因此西北大学是Stock Center的最自然选择。本提案的目标有两个：(1)通过继续收集盘基骨菌和相关生物的新天然分离株、产生表型的突变体、标记菌株以及cDNA文库和抗体等宝贵资源来维持和扩大库存中心的馆藏；(2)将库存中心的资料分发给研究界。迪迪库存中心已经建立了接收、测试和储存菌株和质粒的标准操作程序，我们将致力于继续执行这些政策和程序，因为它们在为社区提供服务方面非常成功。公共卫生相关性：真核生物盘基骨柱为生物医学、遗传和环境领域的研究提供了一个简单的实验系统。重建迪迪库存中心现有的大量菌株所需的资源远远超过储存它们所需的资源。Dictyostelium中央库存中心的存在确保了整个研究社区所有重要菌株的可用性，从而使使用这种生物的所有研究更具成本效益。它还为研究人员提供了一种有效和独立的手段，以遵守NIH关于共享基于模式生物的研究材料的政策。

DESCRIPTION (provided by applicant): The simple eukaryote Dictyostelium discoideum is a model organism widely used for the study of cellular processes such as cell motility, chemotaxis, cellular differentiation, signal transduction, the molecular basis of human diseases, the response to drugs and the cellular mechanisms of drug resistance. As a soil dweller that must compete and defend itself against other micro-organisms, it produces a vast array of secondary metabolites that could be of medical interest. Its ability to recognize itself from other cells with which it bears varying degrees of genetic relationship makes it an fascinating model for the study of self/non-self recognition in microbial ecosystems. Moreover, its phylogenetic position at the intersection between plants and animals makes it a useful organism to include in comparative genomics studies. All those studies require the use of strains, either wild type variants or genetically modified. Since 2002, the Dictyostelium research community has benefited from the presence of a central Stock Center. This resource provides a cost-effective means of ensuring that strains are available and maintained in standardized conditions. The Stock Center is currently overseen by Richard Kessin and Jakob Franke (Columbia University) who will retire at the end of the current funding period. This application seeks support to carry on collecting the known species of social amoebae and isolates of well-studied species like D. discoideum and D. purpureum, in addition to genetically modified strains that have interesting phenotypic characteristics. This provides a great opportunity to move the Stock Center and the Dictyostelium discoideum genome resource, dictyBase, closer together. Northwestern University is the host for dictyBase, making Northwestern the most natural choice for housing the Stock Center. The goals of this proposal are twofold: (1) maintain and expand the Stock Center collection by continuing to collect new natural isolates of Dictyostelium and related organisms, mutants that produce phenotypes, marked strains, as well as valuable resources such as cDNA libraries and antibodies; and (2) distribute the materials from the Stock Center to the research community. The Dicty Stock Center has established standard operating procedures for receiving, testing and storing strains and plasmids that we will commit to continuing these policies an procedures as they have been very successful in providing service to the community. PUBLIC HEALTH RELEVANCE:The eukaryote Dictyostelium discoideum provides a straightforward experimental system for research in the biomedical, genetic, and environmental domains. The resources necessary to reconstruct the large number of strains available at the Dicty Stock Center far exceed those of storing them. The presence of a central Stock Center for Dictyostelium makes all research using this organism more cost-effective by ensuring the availability of all important strains to the entire research community. It also provides researchers an efficient and independent means of complying with NIH policies on sharing of materials from model organism based research.