Spinning Disk Confocal Microscope

描述(由申请人提供)：西奈山医学院的研究人员需要一台可用于长期活细胞成像的旋转圆盘共聚焦显微镜。主要用户将使用该仪器研究人类免疫缺陷病毒(HIV)的细胞间传播、神经元动态平衡、维甲酸受体与乳腺癌、细胞核酸摄取以及血小板形成机制。该仪器将放置在显微镜共享研究设施中并由其进行维护，该设施目前正在为100多名西奈山调查人员的研究服务。该设施的两名全职生物医学工程师将为所要求的仪器的用户提供监督和培训，并负责其维护。公共卫生相关性：所要求的仪器将支持病毒学、神经学、肿瘤学和血液学关于人类免疫缺陷病毒(HIV)、神经细胞生物学、乳腺癌中的维甲酸受体、细胞的核酸摄取和血小板形成的研究。产生的信息可能会影响对艾滋病、乳腺癌和神经退行性疾病等重大人类健康问题的治疗，并可能对各种基因治疗策略产生广泛影响。

DESCRIPTION (provided by applicant): A spinning disk confocal microscope equipped for long term live cell imaging is requested for the use of researchers at the Mount Sinai School of Medicine. Major users will use the instrument for studies intercellular transmission of human immunodeficiency virus (HIV), neuronal homeostasis, retinoic acid receptors and breast cancer, cellular nucleic acid uptake, and mechanisms of platelet formation. The instrument will be placed in and maintained by the Microscopy Shared Research Facility, an ongoing operation currently serving the research of more than one hundred Mount Sinai investigators. The facility's staff of two full time biomedical engineers will provide supervision and training for users of the requested instrument and will be responsible for its maintenance. PUBLIC HEALTH RELEVANCE: The requested instrument will support research in virology, neurology, oncology, and hematology concerning human immunodeficiency virus (HIV), neuronal cell biology, retinoic acid receptors in breast cancer, nucleic acid uptake by cells, and the formation of blood platelets. The information generated could impact therapies for major human health problems including AIDS, breast cancer and neurodegenerative disorders and could have broad implications for a variety of gene therapy strategies.