Spinning Disk Confocal Microscope

描述（由申请人提供）：要求为西奈山医学院的研究人员提供一台用于长期活细胞成像的旋转盘共聚焦显微镜。主要用户将使用该仪器研究人类免疫缺陷病毒（HIV）的细胞间传播、神经元稳态、视黄酸受体和乳腺癌、细胞核酸摄取和血小板形成机制。该仪器将放置在显微镜共享研究设施中并由其维护，该设施目前正在为西奈山100多名研究人员提供研究服务。该设施的两名全职生物医学工程师将为所要求的仪器的用户提供监督和培训，并负责仪器的维护。公共卫生相关性：所申请的仪器将支持有关人类免疫缺陷病毒（HIV）的病毒学、神经学、肿瘤学和血液学的研究，神经细胞生物学，乳腺癌中的视黄酸受体，细胞对核酸的摄取和血小板的形成。所产生的信息可能影响主要人类健康问题的治疗方法，包括艾滋病、乳腺癌和神经退行性疾病，并可能对各种基因治疗策略产生广泛影响。

DESCRIPTION (provided by applicant): A spinning disk confocal microscope equipped for long term live cell imaging is requested for the use of researchers at the Mount Sinai School of Medicine. Major users will use the instrument for studies intercellular transmission of human immunodeficiency virus (HIV), neuronal homeostasis, retinoic acid receptors and breast cancer, cellular nucleic acid uptake, and mechanisms of platelet formation. The instrument will be placed in and maintained by the Microscopy Shared Research Facility, an ongoing operation currently serving the research of more than one hundred Mount Sinai investigators. The facility's staff of two full time biomedical engineers will provide supervision and training for users of the requested instrument and will be responsible for its maintenance. PUBLIC HEALTH RELEVANCE: The requested instrument will support research in virology, neurology, oncology, and hematology concerning human immunodeficiency virus (HIV), neuronal cell biology, retinoic acid receptors in breast cancer, nucleic acid uptake by cells, and the formation of blood platelets. The information generated could impact therapies for major human health problems including AIDS, breast cancer and neurodegenerative disorders and could have broad implications for a variety of gene therapy strategies.