Microscopy

显微镜核心的作用是提供一个集中的设施来服务于电子显微镜和 计划项目建议书中所有研究人员的荧光显微镜需求。这一集成 设施符合成本效益，并有助更有秩序和有效率地使用显微镜资源，以完成 及时提出项目建议。根据期间该设施的使用情况，需要适度的预算 目前的授权期，以使设施能够得到适当的维护和平稳运行。 按照三个项目的常规要求，核心的具体职能将包括： (1)电子显微镜样品制备 (2)对病毒、重组颗粒和病毒-碳水化合物复合体进行EM成像以评估纯度， 颗粒完整性和浓度 (3)杯状病毒感染动物和人类粪便样本的电磁成像 (4)对染色和固定的病毒感染细胞薄片进行EM成像，以评估细胞结合 (5)使用金标记物的免疫电子显微镜 (6)电子显微镜数据的采集、共享和归档 (7)EM的调度和日常维护 (8)荧光显微镜、共聚焦显微镜和去卷积显微镜的样品制备 (9)安排和日常维护荧光、共焦和去卷积显微镜 10)使用荧光、共焦和反卷积显微镜进行数据采集和解释

The role ofthe Microscopy Core is to provide a centralized facility to serve the electron microscopy and fluorescence microscopy needs of all ofthe investigators in the Program Project Proposal. This integrated facility is cost-effective and facilitates more orderly and efficient use of microscopy resources to complete the proposed projects in a timely fashion. A modest budget is requested, based on the use of this facility during the current grant period, to enable proper maintenance and smooth operation of the facility. The specific functions ofthe Core, as required routinely by the three projects, will include: (1) Specimen preparation for electron microscopy (2) EM imaging of viruses, recombinant particles, and virus-carbohydrate complexes to evaluate purity, particle integrity, and concentration (3) EM imaging of fecal samples from calicivirus-infected animals and humans (4) EM imaging of stained and fixed thin sections of virus-infected cells to assess cell binding (5) Immune electron microscopy using gold labels (6) Data acquisition, sharing, and archiving from the electron microscope (7) Scheduling and day-to-day EM maintenance (8) Specimen preparation for fluorescent, confocal and deconvolution microscopy (9) Scheduling and day-to-day maintenance of the fluorescent, confocal and deconvolution microscopes 10) Data acquisition and interpretation using fluorescent, confocal and deconvolution microscopes