Protein & Small Molecule Chemistry Core

蛋白质和小分子化学核心(核心C)将提供与三个主要项目所需的病毒蛋白质和病毒样颗粒(VLP)的克隆、表达和纯化有关的仪器、专业知识和服务。此外，核心将为项目1和项目3中提出的小分子抑制剂发现工作提供化学合成支持。该核心将提供以下具体功能：(1)将编码病毒蛋白的野生型和突变基因克隆到细菌和昆虫表达系统中；(2)病毒蛋白的纯化；(3)VLP的表达、纯化和表征；(4)与抗体、多糖和小分子的相互作用的量化；(5)项目1中提出的用于诊断分析的多肽阵列的合成；(6)合成小分子，以检查结构-活性关系，并提高项目1和3中开发的候选分子的效力。

The Protein and Small Molecule Chemistry Core (Core C) will provide instrumentation, expertise and service related to the cloning, expression and purification of viral proteins and virus-like-particles (VLPs) as needed by each of the three major projects. In addition, the Core will provide chemical synthesis support for the small molecule inhibitor discovery efforts proposed in Projects 1 and 3. This core will provide the following specific functions: (1) cloning of wild-type and mutant genes encoding viral proteins into bacterial and insect expressions systems; (2) purification of viral proteins; (3) expression, purification and characterization of VLPs; (4) quantitation of interactions with antibodies, polysaccharides and small molecules; (5) synthesis of peptide arrays for diagnostics assays proposed in Project 1; (6) synthesis of small molecules to examine structure-activity relationships and increase potency of candidate molecules developed in Projects 1 and 3.