SCIENCE AND RESEARCH CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 科学研究核心为INBRE研究人员和学生提供以下领域的服务和设施：DNA测序和分析，基因表达，生物成像，细胞培养和电生理学，包括卵母细胞表达。 核心小组为个人提供培训和课程培训; DNA测序服务由核心工作人员提供。 2008年6月，许多核心设施被合并到一个新的15,000平方英尺的实验室大楼的两层。 目标是在每个核心领域保持最先进的仪器和材料资源。 除机构间重建研究所外，还寻求其他供资机会，以加强和扩大机构间重建研究所的支助。 在过去的一年里，收到了外部资金，以增加大量新的表达序列标签到一个已经相当大的数据库，为美国龙虾。 微阵列应用目前由至少三个非INBRE授权机构资助，包括NSF，NIH和大西洋加拿大机会局。 包括标准化cDNA文库、文库克隆和BAC在内的资源免费提供给INBRE研究人员，而INBRE以外的研究人员只收取象征性费用。 所有表达的序列标签（EST）在产生时提交给NCBI。 自2004年以来，已公布了六个海洋物种的近100 000份无害环境技术。 微阵列是提供印刷成本。 在INBRE计划的下一年，科学研究核心将继续为缅因州INBRE社区以及那些可以从其设施和资源中受益的计划之外的人提供高效和具有成本效益的服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Science Research Core provides services and facilities to INBRE investigators and students in the following areas: DNA sequencing and analysis, gene expression, biological imaging, cell culture, and electrophysiology including oocyte expression. The core offers training for individuals and in courses; for DNA sequencing the service is provided by core staff. In June 2008, many of the core facilities were consolidated on two floors of a new 15,000-square-foot laboratory building. The goal is to maintain state-of-the-art instrumentation and material resources in each of the core areas. Funding opportunities in addition to INBRE are sought to augment and extend INBRE support. In the past year, external funding was received to add a large number of new expressed sequence tags to an already substantial database for the American lobster. Microarray applications are currently funded by at least three non-INBRE granting agencies, including NSF, NIH, and the Atlantic Canada Opportunities Agency. Resources including normalized cDNA libraries, library clones, and BACs are provided to INBRE researchers at no charge and for a nominal fee to those outside INBRE. All expressed sequence tags (ESTs) are submitted to NCBI as they are produced. Nearly 100,000 ESTs for six marine species have been released since 2004. Microarrays are provided for the cost of printing. In the next year of the INBRE program, the Science Research Core will continue to provide efficient and cost-effective services to the Maine INBRE community as well as those outside the program who can benefit from its facilities and resources.