CELL CULTURE AND HIGH-THROUGHPUT SCREENING CORE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞培养和高通量筛选（HTS）核心设施通过提供在各种条件下进行真核细胞体外研究所需的设备，极大地提高了该机构的生物医学研究能力。自上次审查以来，这一核心设施不仅在规模上，而且在设备和新的研究能力方面都有所扩大。这一扩张导致了六个细胞培养室的创建，额外的细胞储存能力，以及细胞分选和HTS设备的收购。这个核心设施服务于BBRC的所有单位，也吸引了来自各个部门的教师和学生。该设施还增加了调查人员之间的合作次数，加强了对学生和工作人员的培训，并提高了教师的生产力。核心设施对于支持毒物对细胞的影响、细胞免疫反应、细胞-病原体相互作用研究以及许多其他类型的研究至关重要。该设施最近已扩大到包括荧光激活细胞分选仪（FACS），流式细胞仪和HTS成像细胞仪。通过FACS分离细胞群的能力将允许研究者分离和繁殖专门的细胞类型，并进行各种各样的细胞测定，这在过去是不可行的。流式细胞仪可快速分析受多种治疗影响的细胞群，包括分析细胞死亡和分化。HTS成像细胞仪结合了共聚焦显微镜技术，可快速分析高含量培养板。再加上高度复杂的成像软件，机载孵育和机器人平板输送系统，这种多用途设备将用于筛选具有抗病毒，抗癌和抗微生物特性的新型化合物和药物;从而增强BBRC的转化研究能力。随着RCMI的持续资助，预计研究基础设施的这些改善将大大提高个别研究人员的研究能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Culture and High Throughput Screening (HTS) Core Facility has vastly improved the biomedical research capabilities of this institution by providing easy access to equipment that is essential for in vitro studies of eukaryotic cells under a wide variety of conditions. Since the last review, this core facility has been expanded not only in size but also in equipment and new research capabilities. This expansion resulted in the creation of six cell culture rooms, additional cell storage capabilities, and the acquisition of cell sorting and HTS equipment. This core facility serves all the units of the BBRC and has also attracted faculty and students from various departments. The facility has also increased the number of collaborations between investigators, enhanced the training of student and staff, and has increased the productivity of the faculty. The core facility is essential for the support of research on the effects of toxicants on cells, cellular immune responses, studies on cell-pathogen interactions, and many other types of studies. This facility has recently been expanded to include a Fluorescence Activated Cell Sorter (FACS), a Flow Cytomere and a HTS Imaging Cytomer. The ability to separate cell populations by FACS will allow investigators to isolate and propagate specialized cell types and perform a wide variety of cellular assay that were not feasible in the past. The Analytical Flow Cytometer allows for the rapid analysis of populations of cells affected by a wide variety of treatments including analysis of cell death and differentiation. The HTS Imaging Cytometer incorporates confocal microscopy for the rapid analysis of high-content culture plates. Coupled with highly sophisticated imaging software, onboard incubation and a robotic plate delivery system, this multi-purpose equipment will be used to screen novel compounds and drugs with antiviral, anticancer and anti-microbial properties; thus enhancing the translational research capabilities of the BBRC. With continued RCMI funding, it is expected that these improvements in research infrastructure will result in a significant enhancement of the research capabilities of individual investigators.