DEVELOPMENT OF SPECIFIC MICROSPHERE-BASED PROTEASE ASSAYS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项合作是为了开发在UNM的Hypercell平台上使用的高通量蛋白酶分析。在LANL，我们正在开发基于微球的蛋白酶分析，这种方法使用附着在微球上的荧光底物，并通过常规的流式细胞术进行分析。在UNM，我们正在将这些分析整合到Hypercell平台中，用于高通量测量和化学文库筛选。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This collaboration is an effort to develop high throughput protease assays for use on the Hypercyte platform at UNM. At LANL we are developing microsphere based protease assays that use fluorescent substrates attached to microspheres and analyzing them by conventional flow cytometry. At UNM we are incorporating those assays into the Hypercyte platform for high throughput measurements and chemical library screening.