Sample Distribution, Processing and Culture Core

有效的组织获取和利用对于有意义的转化研究活动至关重要。核心 将与每个AML-P01项目以及生物统计和数据管理核心合作， 以及高度协调的患者衍生材料的采购、使用和储存。 样本分配和处理组件，A部分。核心有以下目标： 1.建立和维护血液和骨髓标本库，包括完整细胞、血清， 细胞DNA、RNA和蛋白质，来自血液恶性肿瘤患者，包括 新诊断，缓解和/或复发，接受护理或在医学博士评估。安德森癌症 中心将组织标本分发给AML-P01研究者进行分析，并提供 AML-P01项目中对组织切片进行的研究的解释 AML-P01项目中使用的血液和骨髓样品的组织学表征。 2.维护一个全面的、前瞻性的、交互式的在线数据库， 由核心接收的样本的数据。 3.通过共享血液和骨髓促进AML-P01之间和AML-P01之外的合作 资源 细胞培养核心B部分的具体目标是为本计划项目提供细胞培养支持。 使用我们的组织培养试验，我们可以生长早期和成熟的白血病以及正常的造血细胞。 祖先此外，与核心B合作，我们使用荧光激活细胞分选仪（FACS） 分离罕见的正常细胞和白血病细胞，研究各种抗白血病药物的作用 以及这些细胞的体外生长特性。这些试验非常适合于研究 一号警局调查的各种探员

Effective tissue procurement and utilization is vital for meaningful translational research activities. The Core will work with each AML-P01 project and the Biostatistics and Data Management Core to ensure efficient and highly-coordinated procurement, use and storage of patient derived material. The Sample Distribution and Processing component, part A. Core has the following objectives: 1. Develop and maintain a repository of blood and bone marrow specimens, including intact cells, serum, cellular DMA, RNA and protein, from patients with a hematologic malignancy, including patients who are newly diagnosed, in remission and/or in relapse, receiving care or evaluation at M.D. Anderson Cancer Center. Distribute tissue specimens to AML-P01 investigators for analysis and provide expertise in the interpretation of studies performed on tissue sections within AML-P01 projects Provide comprehensive histological characterization of blood and marrow samples used in AML-P01 projects. 2. Maintain a comprehensive, prospective, interactive online database with detailed clinical and pathologic data for the samples received by the core. 3. Facilitate inter-AML-P01 and extra-AML-P01 collaborations through sharing of blood and marrow resources The specific aim of the cell culture core, Part B is to provide cell culture support for this Program Project. Using our tissue culture assays we can grow early and mature leukemic as well as normal hematopoietic progenitors. Furthermore, in collaboration with Core B, we use a fluorescence-activated cell sorter (FACS) to isolate rare populations of normal and leukemia cells and study the effect of various anti-leukemic agents and the in vitro growth characteristics of these cells. These assays are well suited to study the effects of various agents investigated in this PO1.