2010 Single-Molecule Approaches to Biology Gordon Research Conference

描述（由申请人提供）：本提案要求对2010年6月27日至7月2日在意大利卢卡（巴尔加）Il Ciocco举行的戈登研究会议系列中题为“单分子生物学方法”的国际会议提供部分支持。单分子方法避免系综平均和捕获瞬时中间体和异质行为的能力使得它们在阐明生物学核心分子机器的机制方面特别强大。通过观察个体，可以更深入地了解它们在做什么，它们如何单独工作，它们如何协同工作，以及它们如何在活细胞内工作。本次会议旨在将分子和细胞生物学领域的顶级专家与单分子测量和操作领域的创新者聚集在一起。目标是更好地促进单分子方法的使用，产生新的生物学知识，并更好地为单分子技术的开发和实施提供信息，以解决紧迫的生物学问题。本次会议的具体目标是召集40位代表单分子研究新方向的高级和新兴发言人，在相对孤立的环境中举行为期五天的会议，共有170名与会者。该计划将涵盖单分子测序，DNA/RNA/蛋白质相互作用，折叠机，细胞生物物理学，合成生物学和生物工程，力谱学，新方法开发，细胞超分辨率成像和单分子成像的新型探针的前沿发展。此外，四个海报会议将为与会者提供各种机会，积极参与科学讨论。这一应用的意义在于，戈登研究会议单分子生物学方法是一个强大的工具，以促进和加强新兴/创新方法和重要的生物学问题之间的交流。这一应用与健康相关，因为单分子方法提供了以前无法获得的关于对包括人类病原体在内的所有生命形式至关重要的基本生物化学和生物物理过程的数据，从而扩展了我们的知识，并产生了改善人类健康的新方法。 公共卫生相关性：本申请的健康相关性源于以下事实：生物学的单分子方法提供了以前无法获得的关于对所有生命形式（包括人类病原体）至关重要的基本生物化学和生物物理过程的数据，从而扩展了我们的知识并产生了改善人类健康的新方法。

DESCRIPTION (provided by applicant): This proposal requests partial support for an international conference titled "Single-Molecule Approaches to Biology" as part of the Gordon Research Conference series to be held at Il Ciocco, Lucca (Barga), Italy, June 27 - July 2, 2010. The ability of single-molecule approaches to avoid ensemble averaging and to capture transient intermediates and heterogeneous behavior renders them particularly powerful in elucidating mechanisms of the molecular machines central to biology. By observing individuals, it is possible to more deeply understand what they do, how they work individually, how they work together, and finally, how they work inside live cells. This conference seeks to bring together top experts in molecular and cell biology with innovators in the measurement and manipulation of single molecules. The goals are to better promote the use of the single-molecule approach, to generate new biological knowledge, and to better inform the development and implementation of single- molecule techniques for compelling biological problems. The specific aims of this meeting are to convene 40 senior and emerging speakers who represent new thrusts of single-molecule research with a total of 170 participants for a five day conference in a relatively isolated setting. The program will cover cutting-edge developments in single-molecule sequencing, DNA/RNA/protein interactions, folding machines, cellular biophysics, synthetic biology and bioengineering, force spectroscopy, new method developments, super resolution imaging in cells, and novel probes for single-molecule imaging. In addition, four poster sessions will give participants various opportunities to actively participate in the scientific discourse. The significance of this application is that the Gordon Research Conference on Single-Molecule Approaches to Biology is a powerful vehicle to promote and enhance the exchange between emerging/innovative methods and important biological problems. The health-relatedness of this application derives from the fact that single-molecule approaches provide previously unobtainable data on fundamental biochemical and biophysical processes that are essential for all forms of life, including human pathogens, thus extending our knowledge and generating new approaches to improving human health. PUBLIC HEALTH RELEVANCE: The health-relatedness of this application derives from the fact that single- molecule approaches to biology provide previously unobtainable data on fundamental biochemical and biophysical processes that are essential for all forms of life, including human pathogens, thus extending our knowledge and generating new approaches to improving human health.