MATURATION INTERMEDIATES OF A T=4 VIRUS CAPSID STUDIED BY TIME-RESOLVED X-RAY SC

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 角裸尾虫ω病毒样颗粒（T=4病毒衣壳）经历pH促进的成熟过程。使用SSRL BL 4 -2上的完全升级的仪器，我们已经开始研究与成熟过程相关的结构变化的动力学。 进行手动混合，然后进行一系列时间分辨数据采集（每次3秒暴露），我们最近已经能够在中性（pH 7.6）和pH 5.0之间的中间pH值下分离出结构中间体，该中间体用于促进我们早期研究中的成熟。我们还观察到两种不同RNA含量的VLP的形状和直径略有不同。 我们已经开始了时间分辨的研究与毫秒级的时间分辨率的成熟的整个过程中，试图检验我们的假设，在3倍和准3倍的衣壳亚基之间的静电排斥力是在成熟的关键触发事件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nudaurelia capensis omega virus-like particle, a T=4 virus capsid, undergoes a pH promoted maturation process. Using the fully upgraded instrumentation on SSRL BL4-2 we have begun studying the kinetics of structural change associated with the maturation process. Performing manual mixing, followed by a series of time-resolved data acquisition (each 3 sec exposure), we have recently been able to isolate a structural intermediate at intermediary pH values between neutral (pH 7.6) and pH 5.0, which was used to promote the maturation in our earlier studies. We also observed that two VLPs with different RNA contents show slightly different shape and diameter. We have initiated the time-resolved studies of the entire process of maturation with a millisecond time-resolution in an attempt to examine our hypothesis that the repulsive electrostatic forces among the capsid subunits at the 3-fold and quasi 3-fold axies being the key triggering event in the maturation.