BRIDGES for Evaluation of Health Outcomes, Repercussions and Impacts in Zones of

描述(由申请人提供)：拟议项目的目标是在2010年深水地平线漏油事件之前、期间和之后在墨西哥湾使用被动采样器。被动采样器是作为超级基金中心拨款的一部分开发的用于测量环境应激源(桥梁)的生物反应指示器。这些装置是连接环境暴露和生物反应/影响的空气和水被动采样装置(PSD)。它们可以提供高吞吐量的样本和快速的数据周转。它们隔离多环芳烃(PAHs)、含氧多环芳烃(Opahs)以及其他有机污染物。与多环芳烃相比，多环芳烃可能更具移动性、生物利用度和/或持久性。越来越多的证据表明，欧帕斯具有重要的毒理学意义。增加生物利用度的分散剂，再加上暴露在紫外线下的部位，可能会增加opahs。使用补充的生物分析工具来量化多环芳烃的生物利用度，对于了解化学品泄漏风险等灾害很重要。具体目标1：在撞击之前、期间和之后在墨西哥湾区域部署一套桥梁、空气和水生物分析工具，以定量隔离生物可用污染物。具体目标2：对私营部门司提取物中的成分和污染源类型进行歧视性模式识别和多元回归评估。具体目标3：利用斑马鱼发育模型和Ames试验测试撞击前、撞击期间和撞击后深水地平线溢流桥提取物的相对效力。从翻译的角度来看，目前在含氧多环芳烃和风险评估过程方面存在着重大的数据差距，目前只有空气中的16种优先多环芳烃是环保局的重点。欧帕斯已知是重要的危险，检测和量化将提供关于目前未被捕获的泄漏复杂性的新的和非常重要的信息。外联活动将与该项目合作，将适用于墨西哥湾漏油研究的环境健康教育联系起来，并利用包括网络在内的新方法向在墨西哥湾沿岸各州工作和生活的个人传播。 与公共健康相关：从洛杉矶、密苏里州、阿勒冈州和佛罗里达州的深水地平线漏油事件中快速生成空气和水中的高通量生物可用多环芳烃数据将具有独立的价值。首先，创建生物可利用的含氧多环芳烃数据对于了解对环境和人类健康的影响将是非常宝贵的。使用新的方法通过网络提供相关的PAH卫生信息将填补社区领导人和公共卫生专业人员所需的已确定的信息空白。

DESCRIPTION (provided by applicant): The goals of the proposed project will be to use passive samplers in the Gulf of Mexico before, during and post impact from the Deepwater Horizon Spill of 2010. The passive samplers are biological response indicator devices for gauging environmental stressors (BRIDGES) developed as part of a Superfund Center grant. The devices are air and water passive sampling devices (PSD) that bridge environmental exposure and biological response/effect. They can provide for high throughput samples and rapid turnaround of data. They sequester polycyclic aromatic hydrocarbons (PAHs), oxygenated PAHs (OPAHs) as well as other organic contaminants. OPAHs are potentially more mobile, bioavailable and/or persistent than PAHs. There is growing evidence that OPAHs have important toxicological significance. Dispersants that increase bioavailability coupled with sites exposed to UV may increase OPAHs. The use of complementary bioanalytical tools that quantify bioavailability PAH processes is important to understand disasters such as chemical spill risk. Specific Aim 1: Deploy a set of BRIDGES, air and water bio-analytical tools, regionally in the Gulf of Mexico before, during and post impact to quantitatively sequester bioavailable contaminants. Specific Aim 2: Develop discriminatory pattern recognition and multivariate regression assessments of components in PSD extracts and contaminant source type. Specific Aim 3: Utilize the zebrafish developmental model and Ames assay to test the relative potency of pre-, during and post-impact Deepwater Horizon spill BRIDGES extracts. From a translational perspective, a significant data gap currently exists with respect to oxygenated PAHs and the risk assessment process, currently only the 16 priority PAHs in air is EPA's focus. OPAHs are known to be important hazards, the detection and quantification will provide new and very important information on the complexity of the spill that is not currently being captured. The Outreach, in collaboration, will relate environmental health education that applies to the Gulf of Mexico Oil Spill research of the Project and disseminate to individuals working and living in the states along the coastline of the Gulf of Mexico using novel methods including the web. PUBLIC HEALTH RELEVANCE: Rapid generation of high throughput bioavailable PAHs data in air and water from the Deepwater Horizon Spill in LA, MS, AL, and FL will have standalone value. First creation of bioavailable oxygenated PAH data will be invaluable to understanding effects on environmental and human health. Providing pertinent PAH health information to be delivered using novel methods via the web will fill identified information gaps needed by community leaders, and public health professionals.