CORE B - LIGAND ASSAY AND ANALYSIS CORE

作为国家RSB资源，配基分析和分析核心将在U54中发挥重要作用 中心，以及RSB支持的其他中心。首先，核心将提供高质量、高成本效益的检测 服务。第二，开发新的最先进的分析方法，以适应研究人员的需求。第三, 该中心将就化验方法、数据减少/分析、 数据解释和生物数学建模。 生殖激素的准确、准确和可靠的测量是必不可少的 Ligand Core任务中的组件。建立核心设施的理由与 为内部和外部RSB支持的调查人员节省成本、资源和人员，他们将 不需要在每个单独的实验室开发和维护这些方法。技术专长和 由每个调查员执行大容量或不同测试所需的设备将效率低下且 把精力和资源从重要的研究问题上转移开。此外，中央核心设施还可以实施 分析性能、质量控制和数据简化的统一标准。实施新的 方法或对现有方法的改进需要昂贵且低效的技术专业知识 由每个项目调查员建立内部机制。建立核心的主要理由之一是 以经济高效的方式提供最先进的化验服务。这项任务将包括开发 提高灵敏度和特异度的分析方法，从放射性方法转变为非放射性方法 系统、自动化设备的实施(用于大容量测试)。化验自动化可以增强 通过增加检测次数来提高检测精度并降低成本，从而提高检测性能 可以由技术专家执行。最后，向RSB支持的其他中心和其他机构提供服务 弗吉尼亚大学内的生殖科学家，促进合作精神，这可能会导致未来 调查人员之间的合作。

As a National RSB Resource, the Ligand Assay and Analysis Core will serve an essential role in this U54 Center, and other RSB-supported Centers. First, the Core will provide high quality, cost effective assay services. Second, develop new state-of-the-art assay methods to accommodate investigator needs. Third, the Core will provide assistance and consultation regarding assay methodology, data reduction/analysis, data interpretation and biomathematical modeling. The accurate, precise and dependable measurement of reproductive hormones is an essential component in the mission of Ligand Core. The justification for establishing a Core facility relates to the savings in cost, resources and personnel to internal and external RSB-supported investigators, as they will not need to develop and maintain these methods in each individual laboratory. The technical expertise and equipment required to perform high volume or diverse testing by each investigator would be inefficient and divert effort and resources from important research questions. Also, a central Core facility can implement uniform standards for assay performance, quality controls and data reduction. The implementation of new methods or enhancement of current methods require technical expertise that would be costly and inefficient to establish in-house by each project investigator. One of the major rationales for establishing the Core is to provide state of the art assay services in a cost efficient manner. This mission will include development of assays with enhanced sensitivity and specificity, transferring methods from radioactive to nonradioactive systems, implementation of automation equipment (for high volume tests). Assay automation can enhance assay performance by improving assay precision and reducing costs by increasing the number of tests that can be performed by the technologist. Finally, providing services to other RSB-supported Centers and other reproductive scientists within the University of Virginia, promote a spirit of cooperation that may lead to future collaborations between investigators.