Protein Interaction Core

概述 我们的蛋白质交互核心将利用犹他大学现有的核心设施 健康科学核心设施。蛋白质相互作用核心设施成立于1996年，旨在提供 内部和外部学术用户访问最先进的、无标签的实时交互 技术。在过去的10年里，这个机构为100多个研究项目做出了贡献 它涵盖了广泛的生物领域。 蛋白质相互作用核心位于医学院的中心位置(RM4A416) 健康科学校园。该实验室1600平方英尺的空间于1997年进行了彻底翻修 是专门为容纳核心设施的设备和人员而设计的。目前， 该设施维护着7个基于表面等离子体共振的光学生物传感器：3个Biacore 2000，1个Biacore 3000、1个Biacore S51、1个Biacore FlexChip和1个Lumera蛋白质组处理器。Biacore 2000/3000 平台是测量蛋白质-蛋白质相互作用的理想选择，而Biacore S51是为 小分子相互作用分析。FlexChip和Proteome处理器仪器是基于阵列的 成像系统，允许研究固定在传感器上的多达1000个结合伙伴 一次浮出水面。核心与Biacore和Lumera密切合作，开发 这种新的硬件，以及数据分析软件。在2007年初，我们计划增加一个新的生物传感器 (BioRad实验室的ProteOn XPR36)到我们的核心，由ab NIH共享仪器资助 格兰特。总之，这些最先进的仪器使我们能够测量生物分子之间的相互作用 例如高分辨率和高通量格式的蛋白质、寡核苷酸和脂类。 试验是通过与Core经理的电子邮件联系安排的。通常情况下，调查员 然后，他们的工作人员与经理会面，为拟议的研究计划最佳行动方案。 然后样品被送到核心，实验在同一天开始。这确保了 不稳定的样品能够及时处理。

Overview Our Protein Interaction Core will tap into an existing Core facility that is part of the University of Utah's Health Sciences Core facilities. The Protein Interaction Core facility was established in 1996 to provide internal and external academic users access to state-of-the-art, label-free, real-time interaction technologies. Over the past 10 years, this facility has contributed to more than 100 research projects that encompass a wide array of biological areas. The Protein Interaction Core is centrally located within the School of Medicine (Rm 4A416) on the Health Sciences campus. The laboratory's 1600 square feet of space was entirely renovated in 1997 and was specifically designed to accommodate the Core facility's equipment and personnel. Currently, the facility maintains 7 surface plasmon resonance-based optical biosensors: 3 Biacore 2000s, 1 Biacore 3000, 1 Biacore S51, 1 Biacore Flexchip, and 1 Lumera Proteome Processor. The Biacore 2000/3000 platforms are ideal for measuring protein-protein interactions, while the Biacore S51 is designed for small-molecule interaction analysis. The Flexchip and Proteome Processor instruments are array-based imaging systems that allow one to study up to 1000 binding partners immobilized onto the sensor surface at one time. The Core has worked closely with both Biacore and Lumera on the development of this new hardware, as well as data analysis software. In early 2007, we plan to add a new biosensor (the ProteOn XPR36 from BioRad Laboratories) to our Core, funded by ab NIH Shared Instrument Grant. Together, these state-of-the-art instruments allow us to measure the interactions of biomolecules such as proteins, oligonucleotides, and lipids in high-resolution, as well as high-throughput, formats. Experiments are scheduled through email contacts with the Core's manager. Typically, the investigator and their staff then meet with the manager to plan the best course of action for the proposed study. Samples are then dropped off at the Core and experiments are initiated the same day. This ensures that labile samples are able to be processed in a timely manner.