C1 - Biospecimen Repository Core

生物标本库核心（CF-A）旨在为基础生物学研究提供支持 孢子的努力。核心将在收集、注释、存储、分发和 跟踪研究方案中登记的患者的肉瘤和正常组织标本。四个关键 肉瘤孢子生物标本库的重点领域是高质量的库存裂隙（冷冻和 石蜡包埋）和血液（血清和血浆）;准确和更新的程序，病理， 临床注释;用于研究方案的生物样本的高效和有效分发;以及 强大的跟踪系统，能够从收集到使用过程中监控生物标本。整合 这四个组成部分是生物标本库核心的主要目标。 核心将为孢子研究者提供肿瘤样本的专家组织病理学评估， 从参与研究方案的患者以及从小鼠和异种移植模型中。核心将提供 协助进行和解释免疫组织化学研究，选择裂隙， 显微解剖和阵列的构建，并与项目负责人和CF-B合作。芯会 还提供了在人体组织和动物模型中肿瘤对治疗的反应的系统评估， 肉瘤，包括药物反应的免疫组织化学标志物的解释，如 激酶磷酸化、增殖抑制和诱导凋亡。核心将提供 肉瘤肿瘤和细胞系的突变注释，如个别研究项目所需。

The Biospecimen Repository Core (CF-A) is designed to provide support to the basic translafional research efforts of the SPORE. The Core will play a central role in collecfing, annotafing, storing, distribufing, and tracking sarcoma and normal tissue blospecimens from pafients enrolled in research protocols. The four key focus areas forthe sarcoma SPORE Biospecimen Repository are high quality banked fissue (frozen and paraffin-embedded) and blood (serum and plasma); accurate and updated procedural, pathologic, and clinical annotafion; efficient and effective distribufion of blospecimens for use in research protocols; and a robust tracking system capable of monitoring blospecimens from collecfion through utilization. Integrating these four components is a major goal of the Biospecimen Repository Core. The Core will provide SPORE invesfigators with expert histopathologic evaluation of tumor samples, both from pafients enrolled on research protocols and from murine and xenograft models. The Core will provide assistance in performing and interprefing immunohistochemical studies, in selecfing fissue for microdissecfion and construcfion of arrays, and in collaborating with project leaders and CF-B. The core will also provide systematic assessment of tumor response to therapy in human tissues and in animal models of sarcomas, including interpretafion of immunohistochemical markers for drug response, such as changes in kinase phosphorylafion, inhibifion of proliferafion, and inducfion of apoptosis. The Core will provide mutafional annotafion for sarcoma tumors and cell lines, as needed by individual research projects.