Mass Spectrometry and Proteomics Shared Resouce

质谱学和蛋白质组学(MSP)共享资源为亨斯迈癌症研究所(HCI)提供 成员和整个犹他大学研究界提供广泛的质谱学服务， 包括最先进的仪器、咨询和专业知识，特别是基于蛋白质组学的 研究。混合傅里叶变换质谱计(LTQ-FT型，ThermoElectron型)是主要的 蛋白质组学仪器。它提供低至50atmool、高质量的蛋白质识别(ID) 准确度(&lt；2ppm)，以及电子捕获解离(ECD)、红外多光子测序 解离(IRMPD)和碰撞诱导解离(CID)。蛋白质组学服务包括蛋白质ID； 翻译后修饰的特征(例如，磷脂)；以及完整蛋白质的分析。这个 资源还为广泛的其他研究应用提供质谱学服务，例如 天然产物、药物和药物-聚合物结合物、核酸、DNA扩增的结构分析 产品(例如，聚合酶链式反应)、大的生物分子和聚合物、多肽和合成小分子。乍得 Nelson博士自2003年以来一直在两名工作人员的协助下指导MSP共享资源。 MSP共享资源的仪器和工作人员位于中央位置。 MSP共享资源服务于所有基于HCI实验室的科学项目(细胞的核控制 生长和分化；细胞反应和调节；成像、诊断和治疗；以及 胃最终癌症)，分子，特别是蛋白质和翻译后修饰的特征， 在癌症机制中发挥作用。此外，还分享了MSP对小分子化合物的表征 资源，包括天然产品和药物释放聚合物，对 发展疗法对人机界面的激活。 MSP共享资源是一个机构管理的设施，受到大学和HCI的监督 领导力。有一个学院咨询委员会，并通过以下方式审查资源以确保用户满意 年度调查。该设施是按服务收费(按存储容量使用计费)运营的。在2008年，全面使用 MSP由癌症中心成员共享资源，由同行评审的基金和癌症中心共享 资源占31%。要求CCSG提供14%(44,857美元)的资金 建议的资源预算。MSP共享资源有足够的容量供癌症患者额外使用 中心成员。

The Mass Spectrometry and Proteomics (MSP) Shared Resource provides Huntsman Cancer Institute (HCI) members and the entire University of Utah research community a broad range of mass spectrometry services, including state-of-the-art instrumentation, consultation, and expertise, particulariy for proteomics-based research. The hybrid Fourier-Transform mass spectrometer (model LTQ-FT, ThermoElectron) is the main proteomics instrument. It provides protein identification (ID) at levels as low as 50 attomole, high-mass accuracy (<2ppm), and peptide sequencing by electron-capture dissociation (ECD), infrared multiphoton dissociation (IRMPD), and collision-induced dissociation (CID). Proteomics services include protein ID; characterization of post-translational modifications (e.g., phosphorylafion); and analysis of intact proteins. The Resource also provides mass spectrometry services for a wide range of other research applications such as structural analysis of natural products, drugs and drug-polymer conjugates, nucleic acids, DNA amplification products (e.g., PCR), large biomolecules and polymers, pepfides, and small synthetic molecules. Chad Nelson, PhD, has directed the MSP Shared Resource since 2003, with the assistance of two staff members. The instrumentation and staff of the MSP Shared Resource are centrally located. The MSP Shared Resource serves all HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiafion; Cell Response and Regulafion; Imaging, Diagnosfics, and Therapeufics; and Gastrointesfinal Cancers), characterizing molecules, particulariy proteins and post-translational modifications, that play roles in cancer mechanisms. In addition, characterization of small compounds by the MSP Shared Resource, including natural products and drug-delivery polymers, contributes significantly to the developmental therapeufics activifies of HCI. The MSP Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). In 2008, overall use of the MSP Shared Resource by Cancer Center members with peer-reviewed funding and Cancer Center Shared Resources was 31 percent. Funds are requested from the CCSG to cover 14 percent ($44,857) of the proposed Resource budget. The MSP Shared Resource has ample capacity for additional use by Cancer Center members.