TRD2: Functional Imaging

项目摘要 TRD2 TRD项目2将开发新的光学相干层析成像(OCT)技术来解决未满足的问题 在组织功能成像方面的需求。具体目标#1解决了对高速和高速数据传输的需求 用于临床前研究的分辨率血管成像系统。一种新的高速光结构 对于高分辨率光学相干显微镜(OCM)，其速度将比 现有的OCM仪器。这些速度上的改进将被用于高速扩展 分辨率血管成像和定量血流成像扩展到更广泛的成像领域，并提高 这些测量的时间分辨率。具体目标2建立在最近的发展基础上 大深度范围、高速OCT架构，创建术中OCT相机平台。 通过偏振敏感OCT的矢量双折射成像将被整合到相机中 一种系统，允许在手术视野内想象光学双折射。虽然这个平台技术 可以在许多外科手术中应用，它将作为一种手段进行具体验证 周围神经损伤的功能成像。最后，具体目标3解决了以下需求 改进的研究慢性炎症性疾病和囊性病变小动物模型肺功能的工具 纤维化(CF)。为了研究炎症驱动的淋巴重塑对肺功能的影响， 将开发用于对小鼠气管集合淋巴管进行成像的导管和方法 已验证。使用类似的导管和一套新的成像方法，体内平台提供 Cf Will雪貂模型的呼吸道表面液体性质和粘液纤毛转运的测量 被证明。通过这些目标，TRD项目2将开发、验证和交付创新的新技术 向我们协作的项目调查人员和更广泛的研究社区提供OCT技术。

Project Summary TRD 2 TRD project 2 will develop novel optical coherence tomography (OCT) technologies to address unmet needs in the functional imaging of tissue. Specific Aim #1 addresses the need for high-speed and high- resolution angiographic imaging systems for preclinical research. A new high-speed optical architecture for high-resolution optical coherence microscopy (OCM) will be constructed that is 100 times faster than existing OCM instrumentation. These improvements in speed will be leveraged to expand high resolution angiography and quantitative flow imaging to wider imaging fields, and to improve the temporal resolution of these measurements. Specific Aim #2 builds upon the recent development of long depth range, high-speed OCT architectures to create an intraoperative OCT camera platform. Vectorial birefringence imaging through polarization-sensitive OCT will be incorporated into the camera system to allow imagining of optical birefringence across a surgical field. While this platform technology can have application in numerous surgical procedures, it will be specifically validated as a means for functional imaging of peripheral nerve injuries. Finally, specific aim #3 addresses the need for improved tools to study lung function in small animal models of chronic inflammatory disease and cystic fibrosis (CF). To study the consequences of inflammation-driven lymphatic remodeling on lung function, catheters and methods to image the collecting lymphatics of the mouse trachea will be developed and validated. Using similar catheters and a new set of imaging methods, an in vivo platform providing measurements of airway surface liquid properties and mucociliary transport in ferret models of CF will be demonstrated. Through these aims, TRD project 2 will develop, validate, and deliver innovative new OCT technologies to our collaborating project investigators and to the broader research community.