Cryogenic freezer chest for sample storage

所要求的低温冷冻箱可在-150℃下储存生物样品。在这个温度下保存样品不需要液氮。在-150℃时，样品内部和外部都不会发生冰再结晶，因此样品的生物活性被阻止。样品可以在以后的时间进行分析或重新分析，以验证数据的可重复性。所要求的设备是一种通用工具，可以长时间储存生物样品。从燃料酒精生产过程中收获的酵母样品将储存在要求的冷冻室中。这些样品将用于进行全细胞基因表达和蛋白质表达谱分析，试图探测酵母在双重渗透（由于高糖浓度）和抑制（由于高乙醇水平）作用下的生理变化。因此，可以开发一种控制策略来减轻这种不利影响，为燃料酒精工业设计高乙醇收率工艺。

The requested cryogenic freezer chest can store biological samples at -150 oC. No liquid nitrogen is required to keep stored samples at this temperature. At -150 oC, no ice re-crystallization will occur from within and outside samples and the biological activities of the sample are thus arrested. Samples can then be analyzed at a later time or re-analyzed to validate the data reproducibility. The requested equipment is a general-purpose tool that can store biological samples for an extensive period of time. Yeast samples harvested from a fuel alcohol production process will be stored in the requested freezer. These samples will be used to carry out whole-cell gene expression and protein expression profiling, attempting to probe yeast physiological variations under a dual osmotic (due to high sugar concentration) and inhibitory (due to high ethanol level) effect. Consequently, a control strategy can be developed to alleviate this adverse effect, toward the designing of a high ethanol-yield process for fuel alcohol industry.