Microbial and cell culture analytical system

提议的设备是台式流式细胞仪（Guava EasyCyte Mini with Side Scatter Upgrade and Advanced Assay Suite），能够测量细胞浓度、细胞活力、细胞周期期和凋亡。它还能够检测细胞产生的生物分子，如蛋白质。提议的设备将用于测量细胞浓度和活力在一个群体。此外，它将用于根据细胞周期或凋亡的位置将群体中的细胞分配给亚群体，并评估细胞的相对蛋白质产量。生长的细胞群将被安置在拟议的二氧化碳培养箱中，并将储存在拟议的超低温冰箱中。等待台式流式细胞仪分析的样品也将储存在建议的超低温冰箱中。通过使用台式流式细胞仪进行的分析收集的数据将用于开发培养系统模型和开发培养优化策略。从哺乳动物细胞培养中产生的蛋白质代表了一个快速增长的生物制药类别。对于高度可复制的哺乳动物细胞培养系统，高质量的个人和稳健的模型和优化策略仍然是需要的。此外，随着市场的不断扩大，熟练掌握动物细胞技术的HQP日益短缺。拟议的设备使HQP的研究和培训与生物制药工业和一般生物技术工业高度相关。

The proposed equipment, a desk top flow cytometer (the Guava EasyCyte Mini with Side Scatter Upgrade and Advanced Assay Suite), is capable of measuring cell concentration, cell viability, cell cycle phase, and apoptosis. It is also capable of detecting biomolecules, such as proteins, produced by the cells. The proposed equipment will be used to measure cell concentration and viability within a population. Further, it will be used to assign the cells in the population to subpopulations on the basis of position in the cell cycle or in apoptosis, and to assess the relative protein productivity of the cells.    Growing cell populations will be housed in the proposed carbon dioxide incubator and will be stored in the proposed ultra low temperature freezer. Samples awaiting analysis with the desk top flow cytometer will also be stored in the proposed ultra low temperature freezer.    Data collected through assays performed with the proposed desk top flow cytometer will be used to develop models of the culture system and to develop culture optimization strategies.    Proteins produced from mammalian cell culture represent a rapidly growing class of biopharmaceuticals. Both highly qualified personal and robust models and optimization strategies for highly reproducible mammalian cell culture systems continue to be in demand. Moreover, as the market continues to expand, a growing shortage of HQP skilled in animal cell technology has developed. The research and the training of HQP enabled by the proposed equipment are highly relevant to the biopharmaceutical industry and to the biotechnology industry in general.