Rapid scanning stopped flow spectrometer circular dichroism capability tool and gel electrophoresis-transblot system

将圆二色性（CD）能力添加到现有的快速扫描停流（RS-SF）分光光度计沿着凝胶电泳-transblot系统，对于阿尔伯塔大学土木与环境工程系的我们的研究小组进行朊病毒灭活的研究将是非常有用的并且是迫切需要的。蛋白质感染性颗粒）是一组称为传染性海绵状脑病（TSE）的神经变性疾病如牛海绵状脑病（BSE）的病原体。自2005年1月在加拿大出生的奶牛中首次发生BSE以来，加拿大的养牛业一直在生产大量的特定风险材料（SRM），以尽量减少BSE对人类健康，食品安全和动物健康造成的风险。由于朊病毒蛋白在环境中具有潜在的持久性和独特的稳定性，因此迫切需要开发有效的朊病毒蛋白在简单和复杂基质中的去污方法，臭氧化和高级氧化处理正在测试用于破坏传染性羊瘙痒症朊病毒以及朊病毒模型蛋白。据推测，朊病毒样蛋白的一级和二级结构可以成功地破坏臭氧和AOPs。这一假设可以通过CD光谱法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法进行检验，所述CD光谱法使用现有的RS-SF分光光度计与待购买的新CD模块耦合（SDS-PAGE）和Western印迹分析，使用新的凝胶电泳-transblot系统沿着现有的液相色谱-离子阱串联质谱仪在我们的实验室。这个朊病毒灭活研究计划将最终导致非常有效和经济可行的臭氧和该项目旨在为固体废弃物处置过程中产生的废物提供先进的氧化处理工艺，并将有助于保护加拿大的野生动物、动物饲养业务和公众健康。

A circular dichroism (CD) capability added to an existing rapid scanning stopped flow (RS-SF) spectrophotometer along with a gel electrophoresis-transblot system, will be highly useful and urgently needed for our research group in the Department of Civil & Environmental Engineering at the University of Alberta to conduct research on prion inactivation.Prions (i.e., proteinaceous infectious particles) are causative agents of a group of neurodegenerative diseases called transmissible spongiform encephalopathies (TSEs) such as bovine spongiform encephalopathy (BSE) in cattle. Since the first occurrence of BSE in a Canadian-born cow in January 2005, the cattle industry in Canada has been producing large amounts of specific risk materials (SRMs) to minimize the risks that BSE causes to human health, food safety and animal health. Because of potential persistence and unique stability of prion proteins in the environment, there is an urgent need for developing effective decontamination methods for prion protein in simple and complex matrices.Ozonation and advanced oxidation treatments are being tested for the destruction of infectious sheep scrapie prion, as well as prion-model proteins. It is speculated that the primary and secondary structures of prion-like proteins can be successfully disrupted by ozonation and AOPs. This hypothesis can be tested by CD spectroscopy using an existing RS-SF spectrophotometer coupled with a new CD module to be purchased and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis using the new gel electrophoresis-transblot system along with an existing liquid chromatography-ion trap tandem mass spectrometer in our laboratory.This prion inactivation research program will eventually lead to a development of very effective and economically feasible ozone and advanced oxidation treatment processes for the wastes from SRMs disposal, and will help protect Canadian wildlife, animal feeding operations, and public health.