Molecular tools for drug resistance research

该研究计划的长期目标是了解abc转运蛋白介导的寄生线虫的耐药性，以丝状线虫马来布鲁贾为模式生物。短期目标提出的研究将扩大ABC转运蛋白的基础知识，并将(1)鉴定和分类马来芽孢杆菌中的所有ABC转运蛋白，(2)鉴定它们的组织分布，(3)确定它们在所有生命周期阶段的组成表达，(4)确定它们在药物处理或耐药蠕虫中的表达。马来芽孢杆菌ABC转运体的鉴定和表征需要分子生物学设备，如二维蛋白质电泳系统，冷冻微离心机和梯度热循环器。需要一个二氧化碳培养箱来培养寄生虫。

The long-term goal of the research program is to understand ABC-transporter mediated drug resistance in parasitic nematodes, using the filarial nematode Brugia malayi as the model organism.  The short-term objectives propose research that would expand the basic knowledge of ABC transporters and would (1) identify and classify all ABC transporters in B. malayi, (2) identify their tissue distribution, (3) determine their constitutive expression in all life cycle stages and (4) determine their expression in drug treated or resistant worms.  The identification and characterization of ABC transporters in B. malayi requires molecular biology equipment, such as a two-dimensional protein electrophoresis system, a refrigerated microcentrifuge and a gradient thermal cycler.  A carbon dioxide incubator is required to culture the parasite.