Revealing how transcription factors search the DNA to control preimplantation development in mammals

哺乳动物胚胎的发育依赖于与DNA结合的蛋白质来激活不同的基因。虽然已经确定了几种在胚胎发育过程中调节基因的蛋白质，但这些蛋白质如何找到它们特定的DNA靶标仍然未知。我们将应用新的非侵入性方法来分析正常发育的完整小鼠胚胎中dna结合蛋白的运动，并将确定控制dna -蛋白质相互作用的分子机制。

The development of mammalian embryos relies on proteins that bind to DNA to activate different genes. While several proteins regulating genes during embryonic development have been identified, it remains unknown how these proteins find their specific DNA targets. We will apply new non-invasive methods to analyse the movement of DNA–binding proteins in intact mouse embryos undergoing normal development, and will determine the molecular mechanisms that control DNA–protein interactions.