On-line monitoring and characterization of cell metabolism

该研究计划的重点是开发一个不限于细胞系或生物过程的生物测定平台。以前开发的非侵入性监测工具将结合在一起并进一步开发。将研究植物和哺乳动物细胞，目的是通过提高我们对细胞代谢状态和比生长速率与重组蛋白生产之间联系的理解，提高重组蛋白的细胞比生产率。将使用非侵入性监测工具，例如用于表征细胞代谢和能量的体内核磁共振，以及用于定量分泌的重组蛋白中的培养基浓度的表面等离子体共振。工程还将用于生物医学应用。生物测定平台应能够优化培养基组成、培养条件和策略以及细胞系筛选。最终，培养策略应允许调节细胞代谢、生长和生产力。

The research program focuses on the development of a bioassay platform that is not limited to a cell line or bioprocess. Non-invasive monitoring tools developed previously will be combined together and further developed. Plant and mammalian cells will be studied with the objective of increasing cell specific productivity in recombinant proteins by improving our understanding of the links between cell metabolic state and specific growth rate and recombinant protein production. Non-invasive monitoring tools such as in vivo nuclear magnetic resonance, for the characterization of cell metabolism and energetic, and surface plasmonic resonance, for the quantification of the medium concentration in secreted recombinant protein will be used. Works will also be performed for biomedical applications. The bioassay platform should enable performing optimisation of the culture medium composition, of culture conditions and strategies, and cell line screening. Ultimately, culture strategies should allow to modulate cell metabolism, growth and productivity.