Plant CO2 gas exchange and fluorescence system

叶绿素a荧光与CO2气体交换相结合，现在是评估植物和藻类光合性能和环境胁迫影响的首选技术。Li-Cor 6400 XRT代表了用于此目的的最先进仪器，并将支持植物胁迫和发育一般领域的三个独立研究项目。首先，结合联合收割机测量体内CO2气体交换与伴随测量叶绿素荧光的能力是至关重要的，以阐明机制的调节压力诱导的叶片杂色的模式植物，拟南芥。此外，在体内CO2气体交换和叶绿素荧光测量加上基因表达测量是至关重要的理解特定的谷物品种的驯化，以提高CO2和温暖的温度通常与全球变暖和气候变化的机制。第二，Macfie博士的研究计划侧重于阐明植物对潜在有毒金属离子的反应机制。这包括鉴定操作中的生化和分子机制、相应的信号传导途径以及所涉及的基因或基因家族。金属胁迫引起的生物量积累的变化应反映在光合能力和光合效率的降低。反过来，这应该反映在CO2同化率，PSII光化学效率和能量分配的变化，在体内叶绿素荧光和CO2气体交换测量。第三，亨利博士目前的研究重点是植物和自然生态系统对气候变暖和大气氮沉降的综合影响的反应，这两个生态上重要的全球变化因素预计将在下一个世纪增加。 CO2同化与叶绿素a荧光数据相结合，将有助于预测未来的生产力和温带生态系统的功能，以应对全球环境变化，并将有助于气候变化模型中的大气和生物系统之间的反馈机制框架。

Chlorophyll a fluorescence combined with CO2 gas exchange is now a technique of choice to assess photosynthetic performance and the effects of environmental stress in plants and algae. The Li-Cor 6400XRT represents state-of-the-art instrumentation for this purpose and will support three independent research programmes in the general area of plant stress and development. First, the ability to combine measurements of in vivo CO2 gas exchange with concomitant measurements of chlorophyll fluorescence is crucial to elucidating the mechanism underlying the regulation of stress-induced leaf variegation in the model plant, Arabidopsis thaliana. In addition, in vivo CO2 gas exchange and chlorophyll fluorescence measurements coupled with gene expression measurements are critical to understanding the mechanism underlying the acclimation of specific cereal cultivars to elevated CO2 and warm temperatures typically associated with global warming and climate change. Second, Dr. Macfie's research programme is focussed on the elucidation of the mechanisms by which plants respond to potentially toxic metal ions. This includes identification of the biochemical and molecular mechanism(s) in operation, the corresponding signalling pathways and the genes or gene families involved. Metal stress induced changes in biomass accumulation should be reflected in reductions in photosynthetic capacity and photosynthetic efficiency. In turn, this should be reflected in alterations in CO2 assimilation rates, PSII photochemical efficiency and energy partitioning as measured by in vivo chlorophyll a fluorescence and CO2 gas exchange. Third, Dr. Henry's current research focuses on plant and natural ecosystem responses to the combined effects of climate warming and atmospheric nitrogen deposition, two ecologically important global change factors that are projected to increase over the next century. CO2 assimilation combined with chlorophyll a fluorescence data will help in the prediction of the future productivity and functionality of temperate ecosystems in response to global environmental change, and will contribute to the mechanistic framework underlying feedbacks between the atmosphere and biotic systems in climate change models.