UV/VIS spectrophotometer

申请人要求紫外/可见波长分光光度计，提供快速波长扫描和荧光检测能力，动力学实验的温度控制，以及含有颗粒的样品的混合。应用程序的需要和时机至关重要，因为目前的仪器已经过时和失效，并且由于零件不再可用而无法修复。在我们的实验室里，没有其他仪器，如果它发生故障，会对我们提供研究生培训的能力产生更负面的影响，并推进我们各自的研究领域。这种需求是至关重要的，该仪器是我们研究培训的核心，我们实验室之间的仪器共享将确保它将被广泛用于高质量的个人培训。学生和研究人员将接受分光光度法检测和分析技术的培训，使用荧光标记来跟踪有机分子，酶标记抗体检测技术，化学和酶动力学分析，以及开发原位方法来量化嵌入颗粒中的生物分子。围绕特定仪器的决定是基于积极的供应商经验，强大的技术支持记录，荧光能力，以及在动力学测量和颗粒系统分析过程中混合和控制小体积样品温度的能力。申请者提供基于生物医学研究的研究生培训，包括生物药物的控制递送，新生物材料的开发和组织工程。分光光度计是所有这些研究领域的核心，并且肯定会用于我们所有研究生和研究人员的培训。

The applicants are requesting an Ultraviolet/Visible wavelength spectrophotometer that provides rapid wavelength scanning and fluorescence detection capability, temperature control for kinetic experiments, and mixing for samples containing particulates. The need and timing of the application are critical as the present instrument is obsolete and failing, and cannot be repaired because the parts are no longer available. There is no other instrument in our labs, that if it were to fail, would have a more negative impact on our ability to provide graduate student training, and to advance our respective research areas. The need is critical, the instrument is central to our research training, and the sharing of the instrument between our labs will ensure that it will be used widely in the training of high quality personal. Students and researchers will be trained in spectrophotometric detection and analytical techniques, the use of fluorescent markers to track organic molecules, enzyme labeled antibody detection techniques, chemical and enzymatic kinetic assays, and in the development of in situ methods to quantify biomolecules embedded in particulates. The decision around the specific instrument was based on positive vendor experience, strong record of technical support, fluorescence capability, and the ability to mix and control temperature of small volume samples during analysis for kinetic measurements and for particulate systems. The applicants provide graduate training in biomedically-based research, involving controlled delivery of biopharmaceuticals, development of new biomaterials, and tissue engineering. The spectrophotometer is central to all of these areas of research and will most certainly be used in the training of all of our graduate students and researchers.