Gel documentation and chemiluminescent detection system for use in plant molecular biology

在现代生物化学和分子生物学实验室中，许多DNA、RNA和蛋白质的操作都围绕着这些大分子的凝胶电泳分离。20多年来，CCD成像和信息化记录已经取代了硝酸银薄膜技术的使用。随着这项技术的快速发展，新的成像功能被定期引入。CCD捕获是捕获各种插层染料染色的核酸在荧光激发后发出的光所产生的DNA和RNA凝胶数据的最佳技术。这也可以应用于蛋白质，并且已经开发出特定的染色剂来检测蛋白质，磷酸化蛋白或糖蛋白。有了高分辨率的相机，CCD捕获提供了出版质量的结果，并且随着灵敏度的提高，化学发光等应用现在成为可能。结合后发发光和所需波长的过滤分选，现在可以进行多路复用，并且可以在这种高分辨率的凝胶记录系统上进行2D电泳凝胶分析和斑点定量的比较分析。这里提出的设备主要是为了满足我们日益增长的需求：1)DNA凝胶分析和定量；2)蛋白（Western）印迹化学发光检测；3)荧光在比较和差异蛋白表达分析中的应用。

In modern biochemistry and molecular biology laboratories, numerous DNA, RNA and protein manipulations revolve around gel-based electrophoresis separation of these macromolecules. For more than 20 years now, CCD imaging and informatized record keeping have superseded the use of silver nitrate film based technologies. As this technology is quickly evolving, new imaging capabilities are introduced on a regular basis. CCD capture is the best technology to capture DNA and RNA gel data produced from the emission of light after fluorescent excitation of the nucleic acid stained with various intercalating dyes. This can also be applied to proteins and specific stains have been developed to detect, proteins, phosphoporteins, or glycoproteins. With high-resolution cameras, CCD capture provides publication quality results and with increased sensitivity, application such as chemiluminescene is now possible. Combined with epiluminescence and filter sorting of the desired wavelength, multiplexing is now possible and comparative analysis of, for example, 2D electrophoresis gel analysis and spot quantification can be performed on such gel documentation systems with high resolution. The equipment proposed here is primarily intended to cover our increasing needs in: 1) DNA gel analysis and quantification; 2) chemiluminescence detection from protein (Western) blots; 3) fluorescence applications targeted at comparative and differential protein expression analysis.