Benchtop ultracentrifuge for biochemical isolation and characterization of proteins and subcellular particles

用于蛋白质和亚细胞颗粒的生化分离和表征的台式超速离心机

• 批准号: 359486-2008
• 负责人: Matton, Daniel
• 金额: $ 0.34万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=403702
• 关键词: Benchtop; ultracentrifuge; biochemical; isolation; characterization

Funds are requested to purchase a table top ultracentrifuge and two rotors to process biological samples in the range of 0.2 to 2 mL. This equipment will be used to study plant signal transduction, metabolism, plant reproduction and biological rythms. The ultracentrifuge is the instrument of choice for the purification of plant membranes and the study of protein subcellular localization in vivo and in vitro. An ultracentrifuge able to process very small volumes is critical for the study of small biological samples and plant extracts available in small quantities. This type of instrument is not currently available in our institute and it will support the research activities of approximately 75 graduate students, post doctoral fellows and research professionals.

需要资金购买一台台式超速离心机和两个转子来处理0.2至2毫升范围内的生物样本。 该装置将用于植物信号传导、代谢、植物繁殖和生物节律的研究。 超离心是纯化植物膜和研究蛋白质在体内和体外亚细胞定位的首选仪器。 能够处理非常小体积的超浓缩器对于研究小生物样品和少量植物提取物至关重要。 我们研究所目前没有这种仪器，它将支持大约75名研究生、博士后研究员和研究专业人员的研究活动。