Incubators for cell culture

所要求的仪器将主要用于哺乳动物细胞的受控培养，这是提交申请的研究人员和在生物系工作的其他人的基本程序。该设备将用于在正常生长期间以及暴露于环境和生理压力（包括疾病）时对细胞中的DNA、RNA和蛋白质进行表征。 MacRae博士分析了卤虫胚胎发育和滞育过程中的基因表达和蛋白质合成，滞育是一种生理休眠和增强胁迫耐受性的状态。蛋白质结构/功能的关系和分子伴侣的细胞生存在滞育期间的贡献被认为是在他的工作。Pohajdak博士研究自然杀伤细胞中蛋白质的细胞内转运，这是免疫的主要介质。大部分的工作需要检查蛋白质-蛋白质相互作用，涉及适配器CASP（细胞粘连蛋白相关支架蛋白）与细胞粘连蛋白/ARNO家族的成员相互作用。两个申请人的研究代表了对细胞，分子和发育生物学的基本贡献，并且有许多应用。麦克雷博士的研究涉及分子伴侣在心脏病、白内障和神经系统疾病中的作用。对滞育的研究可以揭示昆虫如何抵抗压力，这在农业和林业中很重要，因为这些生物通常是害虫。对滞育的深入了解将促进卤虫作为水产养殖饲料的使用。Pohajdak博士的研究有助于我们了解免疫系统在识别和消除癌细胞方面的作用。显然，通过使用本文所寻求的孵化器，有可能做出对健康和商业有直接影响的发现。这些设备还将有助于解决许多研究项目的基础问题的实验，从而使申请人在有效利用赠款资金和在几个生物学科进行现代实验的能力方面具有一定的优势。

The requested instrumentation will be used primarily for the controlled culture of mammalian cells, an essential procedure for the researchers submitting the application and for others who work in the Department of Biology. The equipment will be employed for characterization of DNA, RNA and proteins in cells during normal growth and when exposed to environmental and physiological stress, including disease. Dr. MacRae analyzes gene expression and protein synthesis during Artemia embryo development and diapause, a state of physiological dormancy and enhanced stress tolerance. Protein structure/function relationships and the contribution of molecular chaperones to cell survival during diapause are considered in his work. Dr. Pohajdak studies intracellular transport of proteins in natural killer cells, major mediators of immunity. Much of the work entails examination of protein-protein interactions involving the adaptor CASP (Cytohesin Associated Scaffolding Protein) which interacts with members of the cytohesin/ARNO family. The research of both applicants represents fundamental contributions to cell, molecular and developmental biology, and there are many applications. Dr. MacRae's research relates to the role of molecular chaperones in heart disease, cataract, and neurological disorders. Examination of diapause may reveal how insects resist stress, this of importance in agriculture and forestry where these organisms are often pests. A greater understanding of diapause will enhance the use of Artemia as feed in aquaculture. Dr. Pohajdak's research contributes to our understanding of the immune system in recognization and elimination of cancer cells. Clearly, there is potential to make discoveries which have direct consequences to health and commerce through use of the incubators sought herein. The equipment will also facilitate experiments addressing basic questions which are the cornerstone of many research programs, thus placing the applicants at a definite advantage in the effective use of grant funds and the ability to conduct modern experimentation in several biological disciplines.