Integrated bioMEMS (Biological Micro Electro Mechanical System) sensor for real-time heat shock protein 70 monitoring in whole organisms

这项研究的目标是开发一种基于微流体和生物传感器的平台，用于精确和高灵敏度地检测血液中的关键应激相关蛋白--热休克蛋白70（HSP 70）。热休克蛋白是由所有生物体合成的高度保守的蛋白质家族，对正常细胞功能和保护细胞免受应激源至关重要。70千道尔顿质量的HSP（HSP 70）是该蛋白质家族中研究最广泛的成员之一，并且常规地用作细胞应激的指示剂。最近，几项人类研究将HSP 70的血液水平与疾病状态（包括癌症，糖尿病，心血管疾病和创伤）相关联，表明这种应激蛋白测量作为健康受损的生物标志物的实用性。我们还发现，高血清HSP 70水平反映了慢性应激，

The goal of the proposed research is to develop a microfluidics and biosensor-based platform for the precise and highly sensitive detection of a key stress-related protein, the heat shock protein 70 (HSP70), in blood. Heatshock proteins are a family of highly conserved proteins synthesized by all organisms and crucial for normal cellular function and for defending cells against stressors. The 70 kilodalton mass HSP (HSP70) is one of the most widely studied member of this protein family and routinely used as an indicator of cellular stress. Recently, several studies in humans correlated blood levels of HSP70 with disease states, including cancer, diabetes, cardiovascular disease and trauma, suggesting the utility of this stress protein measurement as a biomarker of compromised health. We also found that higher serum HSP70 levels reflect chronic stress in