Fluorescence spectrometer for advanced research in plant physiology, biochemistry and molecular biology

所要求的Perkin-Elmer LS-55荧光光谱仪将用于测量酶活性、测定植物细胞亚细胞区室中的代谢物浓度、测定酶的动力学参数和计算细胞中的代谢通量。该仪器将被放置在纽芬兰纪念大学生物系植物代谢实验室。LS-55荧光光谱仪能够测量各种生物物理/生物化学参数，因此用途非常广泛。它提供了高分辨率的低背景噪声读数，这是非常准确和可重复的，对植物/微生物代谢和生物能量学的最新研究的基本特征。它是研究酶动力学和基于代谢控制理论计算代谢通量的有力工具。这将使我们能够评估在不同条件下应激诱导过程中细胞代谢途径的强度。所要求的设备是大多数生理和生化实验室的组成部分，旨在了解生物能量学中涉及的细胞器，如线粒体和过氧化物酶体的运作。LS-55荧光光谱仪的问世将促进植物和微生物对环境胁迫适应性的研究。这项研究具有重大的生态、工业和进化意义。

The requested Perkin-Elmer LS-55 Fluorescence Spectrometer will be used in the measurement of enzyme activities, determination of metabolite concentrations in subcellular compartments of plant cells, determination of kinetic parameters of enzymes and calculation of metabolic fluxes in the cell. The instrument will be placed in the Laboratory of Plant Metabolism at the Department of Biology, Memorial University of Newfoundland. The LS-55 Fluorescence Spectrometer is capable of measuring diverse biophysical/biochemical parameters and is therefore extremely versatile. It provides high-resolution readouts with low background noise, which are extremely accurate and reproducible, characteristics essential for state of the art research on plant/microbial metabolism and bioenergetics. It is a powerful tool for the investigation of enzyme kinetics and calculation of metabolic fluxes based on the metabolic control theory. It will allow us to evaluate the intensities of cellular metabolic pathways in stress-induced processes under different conditions. The requested piece of equipment is an integral component of most physiological and biochemical laboratories aimed at understanding the operation of cellular organelles involved in bioenergetics, such as mitochondria and peroxisomes. The availability of the LS-55 Fluorescent Spectrometer will promote research on adaptation of plants and microorganisms to environmental stresses. This research has a major ecological, industrial and evolutionary significance.