Gradient themocycler for molecular research on fish neuropeptides

要求提供资金购买一台梯度热循环仪，以取代一台坏了的无法修理的旧仪器。 梯度热循环仪对于Dr.J.D. off维持一个关于调节鱼类食物摄入的生理和内分泌机制的成功研究计划至关重要。 这项研究计划产生的数据将提供新的方法来管理和提高鱼类的生长和繁殖。此外，从长远来看，基于鱼类和高等脊椎动物之间的调节途径的强烈相似性，我们的研究结果将有助于建立脊椎动物摄食内分泌调节的基本模型。这项研究的第一步也是不可避免的一步是通过分子克隆对鱼类大脑食欲调节因子进行表征，这是一个基于使用热循环仪进行核酸扩增的过程。如果不能及时获得这一重要的研究设备，数据生成、研究生和本科生论文工作所需的培训以及出版物无疑会出现延误。

Funds are requested to purchase a gradient thermocycler that will replace an old instrument which broke down and is beyond repair. A gradient thermocycler is absolutely crucial to Dr. Volkoff for the maintenance of a successful research program on the physiological and endocrine mechanisms regulating food intake in fish. Data generated by this research program will provide new approaches to manage and enhance growth and reproduction in fish. In addition, in the long term, based on the strong similarities in the regulatory pathways between fish and higher vertebrates, our results will contribute to the establishment of a fundamental model of the endocrine regulation of feeding in vertebrates. The first and inescapable step in this research consists of the characterization of brain appetite-regulating factors in fish by molecular cloning, a process based on nucleic acid amplification using a thermocycler. Without timely access to this important research equipment, delays in data generation, in training required for both graduate and undergraduate theses work and in publications will undoubtedly occur.