Bacteriophage production using self-cycling fermentation technology

人们对噬菌体（bacteriophage，简称BPHs）的生产越来越感兴趣，BPHs可直接用于噬菌体治疗、诊断和作为抗菌剂，也可用于生产重组蛋白，重组蛋白可用作药物以及农业和食品应用。大规模的生产是困难的。自循环发酵（SCF）是在我的实验室开发的，是一种在同步培养中连续生长微生物的方法。这里提出的工作是一个适应的SCF显着提高生产的CO2。该研究将采用循环策略生产重组蛋白，并最终生产具有重组载体的重组蛋白，并采用一种新的控制策略，简化SCF并优化生产。该系统将开发为两个独立循环的生物反应器。第一阶段用于宿主的生长，并作为正常的自循环发酵罐（SCF）运行。第二阶段用于噬菌体感染宿主，称为自循环感染室（SCIC）。

There is an increasing interest in the production of bacteriophages (phages), which can be used directly in phage therapy, diagnostics and as antibacterial agents and can also be used to produce recombinant proteins, which can be used as pharmaceuticals and in agriculture and food applications. The large scale production of phages is difficult. Self-cycling fermentation (SCF) was developed in my laboratories and is a method of growing microbes continuously, in synchronous culture. The work proposed here is an adaptation of SCF to significantly improve the production of phages. The research will apply a cycling strategy to the production of phages and, eventually, recombinant proteins having phages as vectors and adopt a novel control strategy which simplifies SCF and optimizes the production. The system will be developed as two independently cycling bioreactors. The first stage is used for the growth of the host and operates as a normal self-cycling fermentor (SCF). The second stage is used for the infection of the host by the phage and is called the self-cycling infection chamber (SCIC).