Bio-Rad ChemiDoc MP System

蛋白质在动物和细胞内发生的所有生理和生化过程中起着关键作用。这包括组织生长的调节，神经的结构和功能，以及引起肺部感染的过程。使细胞蛋白质研究复杂化的是，细胞含有数千种不同的蛋白质，每种蛋白质都有多种作用。为了确定哪些蛋白质参与了特定的过程，需要能够识别单个蛋白质的工具，然后表征它们在细胞中的功能。所要求的设备能够识别细胞内的所有蛋白质，并表征不同蛋白质之间的相互作用。例如，该仪器将有助于识别参与调节心脏如何应对压力及其结构和功能变化的蛋白质。这在了解心力衰竭期间人类心脏如何重塑以及开发治疗方法以帮助减少这些变化的影响方面有应用。申请人团队中的每个人都研究具有广泛应用的细胞生物学的一个方面。这包括研究与神经退行性疾病（如多发性硬化症）有关的蛋白质，细胞和动物如何对环境或物理压力作出反应，在囊性纤维化发展过程中人类肺部感染的机制，以及发现新的强效抗生素。这台仪器将使这些研究人员的工作更有效率，并使他们能够完成各种各样的研究，否则是不可能的。因此，这一工具将使这些研究人员能够在其领域取得重大进展，并使发现能够应用于开发针对影响加拿大人健康的各种疾病的新疗法。此外，该仪器对于培训新科学家至关重要，这些新科学家将开发各种生物医学领域（包括药物发现、心血管生物学和疾病传播）急需的技能。

Proteins play critical roles in all physiological and biochemical processes that occur within animals and cells. This includes the regulation of tissue growth, the structure and function of nerves and the processes involved in causing infection of the lungs. Complicating the study of cellular proteins is that cells contain thousands of different proteins and each of these can have multiple roles. To determine which proteins are involved in a specific process requires tools that can identify individual proteins and then characterize how they function in a cell. The requested equipment enables all proteins within a cell to be identified and for the interactions between different proteins to be characterized. For example this instrument will help identify the proteins that are involved in regulating how the heart responds to stress and the changes in its structure and function that result. This has application in understanding how the human heart remodels during heart failure and in the development of treatments to help reduce the effect of these changes. Each of the individuals in the team of applicants investigates an aspect of cellular biology that has broad application. This includes studying the proteins involved in neurodegenerative diseases such as multiple scleroses, how cells and animals respond to environmental or physical stress, the mechanism by which human lungs are infected during the development of cystic fibrosis, and the discovery of new and powerful antibiotics. This instrument will allow the work of these researchers to be more efficient and enable them to complete a variety of studies that would not be otherwise possible. As a result this instrument will enable these researchers to make significant progress in their fields and enable discoveries that will have application in the development of new treatments for a variety of conditions that affect the health of Canadians. In addition, this instrument will be critical to training new scientists who will develop skills that are in significant demand in a variety of biomedical fields including drug discovery, cardiovascular biology and disease transmission.