Econo-efficient formulations of Bacillus thuringiensis based biopesticides

化学农药的不利影响在未来几年变得越来越明显，为生态友好和可持续的生物农药让路。在这种情况下，苏云金芽孢杆菌（Bt）为基础的生物农药统治了几乎95%的世界害虫防治市场。Bt已经使用几种合成和半合成介质进行了大规模生产，然而，原材料成本是限制因素之一，在整体生产中占总生产成本的40%。在这方面，我们的研究实验室已经开发并建立了基于替代介质的工艺，例如淀粉工业废水，用于生产具有高昆虫毒性（昆虫死亡率）潜力的Bt基生物农药。稳定的液体和粉末配方已在实验室规模开发，将在中试规模进一步评估。将这些制剂与工业Bactur 421制剂在昆虫毒性和稳定性方面进行比较。稳定的Bt生物农药（由其保质期研究确定）将在北海岸魁北克省的一个地块上进行中试规模和现场测试，并将结果再次与新开发的工业Bactur 421配方在杀虫时间、杀虫强度、喷雾和应用数量方面进行比较。实地试验将解决技术转让方面的最大差距，简化替代性Bt配方的注册，并提高它们的适销性。该项目将与Milenia公司（巴西）合作进行Bactur 421配方和现场试验，并与积极参与研究的<s:1> <s:1> <s:1> <s:1>昆虫和疾病防治协会（SOPFIM）合作进行。该项目的成功完成将导致替代配方在可销售性和现场有效性方面的结果，并与新的工业Bactur 421配方进行比较。该项目将提供培训HQP的机会（1名博士后和1名研究助理）。

Adverse impacts of chemical pesticides have become evident in the coming years making way for eco-friendly and sustainable biopesticides. In this context, Bacillus thuringiensis (Bt) based biopesticides rule the roost with almost 95% of the world pest control market. Bt has been mass produced using several synthetic and semi-synthetic media, however, cost of raw materials is one of the limiting factors in overall production account for 40 % of the total cost of production. In this regard, our research laboratory has developed and established the process based on alternative media, such as starch industry wastewater for production of Bt based biopesticides with high entomotoxicidal (insect mortality) potential. Stable liquid and powder formulation have been developed at laboratory scale which will be further assessed at pilot scale. The formulations will be compared with the industrial Bactur 421 formulations in terms of entomotoxicity and stability. The stable Bt based biopesticides (as determined by their shelf-life studies) will be developed at pilot scale and field tested in a plot in Quebec in the North coast and the results will be again compared with a newly developed industrial Bactur 421 formulation in terms of insect kill time, insect kill intensity and spray as well as number of applications. The field testing will address the largest gap of technology transfer easing the registration of alternative Bt formulations and enhancing their marketability. The project shall be carried in collaboration with Milenia Inc. (Brazil) for Bactur 421 formulation and field tests in collaboration with la Société de protection des forêts contre les insectes et maladies (SOPFIM), a company which is actively involved in the research. The successful completion of the project will result in getting the outcome on alternate formulations in terms of marketability and field efficacy also when compared with the new industrial Bactur 421 formulations. The project will provide an opportunity to train HQP (one post doc and a research assistant).